238653

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 36109..36845 Replicon plasmid pVA684-146
Accession NZ_CP093460
Organism Klebsiella pneumoniae strain VA684

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag MO416_RS27140 Protein ID WP_003026803.1
Coordinates 36363..36845 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag MO416_RS27135 Protein ID WP_003026799.1
Coordinates 36109..36375 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO416_RS27120 (MO416_27090) 33186..33641 - 456 Protein_31 transposase domain-containing protein -
MO416_RS27125 (MO416_27095) 35169..35474 + 306 WP_227522558.1 CPBP family intramembrane metalloprotease -
MO416_RS27130 (MO416_27100) 35633..35885 - 253 Protein_33 transposase -
MO416_RS27135 (MO416_27105) 36109..36375 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
MO416_RS27140 (MO416_27110) 36363..36845 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
MO416_RS27145 (MO416_27115) 37111..37368 - 258 WP_023292103.1 hypothetical protein -
MO416_RS27150 (MO416_27120) 38010..38525 + 516 Protein_37 CSS-motif domain-containing protein -
MO416_RS27155 (MO416_27125) 38589..39425 + 837 WP_032409249.1 EAL domain-containing protein -
MO416_RS27160 (MO416_27130) 39606..40220 + 615 WP_023316428.1 hypothetical protein -
MO416_RS27165 (MO416_27135) 40280..40408 + 129 Protein_40 transcriptional regulator -
MO416_RS27170 (MO416_27140) 40476..41441 - 966 WP_004118146.1 DMT family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..145622 145622


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T238653 WP_003026803.1 NZ_CP093460:36363-36845 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T238653 NZ_LR890424:c5166146-5165763 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACTTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT238653 WP_003026799.1 NZ_CP093460:36109-36375 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT238653 NZ_LR890424:c5166388-5166146 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References