Detailed information of TA system TA238651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 227466..227991
• Replicon: plasmid pVA684-388
• Accession: NZ_CP093459
• Organism: Klebsiella pneumoniae strain VA684

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A9E1GGX2
• Locus tag: MO416_RS26160
• Protein ID: WP_016946795.1
• Coordinates: 227686..227991 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: MO416_RS26155
• Protein ID: WP_004197642.1
• Coordinates: 227466..227684 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MO416_RS26135 (MO416_26110)	223236..223544	+	309	WP_017896554.1	hypothetical protein	-
MO416_RS26140 (MO416_26115)	224769..225260	-	492	WP_032249453.1	hypothetical protein	-
MO416_RS26145 (MO416_26120)	225394..226626	-	1233	WP_032249456.1	hypothetical protein	-
MO416_RS26150 (MO416_26125)	226623..226871	-	249	WP_004200907.1	hypothetical protein	-
MO416_RS26155 (MO416_26130)	227466..227684	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
MO416_RS26160 (MO416_26135)	227686..227991	+	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	Toxin
MO416_RS26165 (MO416_26140)	228149..228832	+	684	WP_023280892.1	hypothetical protein	-
MO416_RS26170 (MO416_26145)	228835..229611	+	777	WP_049245386.1	site-specific integrase	-
MO416_RS26175 (MO416_26150)	229669..229926	-	258	WP_000764642.1	hypothetical protein	-
MO416_RS26180 (MO416_26155)	230055..230159	-	105	WP_032409716.1	hypothetical protein	-
MO416_RS26185 (MO416_26160)	230694..231560	+	867	WP_004118283.1	replication initiation protein	-
MO416_RS26190 (MO416_26165)	231917..232186	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	formA / tet(D) / sul2 / dfrA8	pla	1..388130	388130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11548.22 Da
• Isoelectric Point: 5.6831

>T238651 WP_016946795.1 NZ_CP093459:227686-227991 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T238651 NZ_LR890424:c4688295-4688011 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT238651 WP_004197642.1 NZ_CP093459:227466-227684 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT238651 NZ_LR890424:c4688527-4688285 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA