238650

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 175698..176608 Replicon plasmid pVA684-388
Accession NZ_CP093459
Organism Klebsiella pneumoniae strain VA684

Toxin (Protein)


Gene name - Uniprot ID A0A2N4Z2R0
Locus tag MO416_RS25845 Protein ID WP_004197882.1
Coordinates 175698..176168 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag MO416_RS25850 Protein ID WP_004181895.1
Coordinates 176165..176608 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO416_RS25815 (MO416_25795) 171678..171890 - 213 WP_032427880.1 hypothetical protein -
MO416_RS25820 (MO416_25800) 172301..172678 + 378 WP_045289277.1 hypothetical protein -
MO416_RS25825 172770..172892 - 123 WP_254911979.1 hypothetical protein -
MO416_RS25830 (MO416_25805) 172876..173850 - 975 WP_004196336.1 hypothetical protein -
MO416_RS25835 (MO416_25810) 174456..174614 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
MO416_RS25840 (MO416_25815) 175328..175588 + 261 WP_004026352.1 hypothetical protein -
MO416_RS25845 (MO416_25820) 175698..176168 - 471 WP_004197882.1 RES family NAD+ phosphorylase Toxin
MO416_RS25850 (MO416_25825) 176165..176608 - 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
MO416_RS25855 (MO416_25830) 176709..177981 - 1273 Protein_174 translesion error-prone DNA polymerase V subunit UmuC -
MO416_RS25860 (MO416_25835) 177981..178403 - 423 WP_004197884.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
MO416_RS25865 (MO416_25840) 178758..179327 - 570 Protein_176 transposase -
MO416_RS25870 (MO416_25845) 179329..179544 + 216 Protein_177 IS200/IS605 family transposase -
MO416_RS25875 (MO416_25850) 180483..181514 - 1032 WP_173018236.1 CRISPR-associated protein Csf2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid formA / tet(D) / sul2 / dfrA8 pla 1..388130 388130
- flank IS/Tn - - 178758..179111 353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17633.92 Da        Isoelectric Point: 4.5152

>T238650 WP_004197882.1 NZ_CP093459:c176168-175698 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDFFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T238650 NZ_LR890424:4625248-4626183 [Klebsiella pneumoniae]
CTGCTTTTTTCTGATGTAACAGATGCTCTTGGTATTGAGTCCGGCGCTATTGTAGAAAAAGATCACTATATCGTTGAGCT
TTTACATTTACTGAAACCGCTAACATTTGACACTCATCAATTGCTGTTTGCCGGAGGAACTGCACTGGCAAAATCGGGCA
TTGTGCTTAACCGAATGTCGGAAGATGTAGATATAAAACTGGTACCCACAGATACTTTCTTGTCTCTTTCGCGCACCCAG
CGCAAAGGTATTCGTAAAGAGATCGTCCAGACAATTTCTCATGCAATAACTGCAACAGACATGTTCAGCTTTGACGATGC
CCATCCAAAAGTGACGCGGGATGAATATCGTTACAACGACATACCCGTTCGCTATCCGCAGCGGGTAGCGCAAGCACCTT
GCCTGCGTCCTTTTATCCAGTTGGAATTGATGGAAGCAAATTTGCTTGAGGCACCAGAGTTCAGGGATATTCATACACTG
GTGACGACACTTACTGGAAAAGCACCTGTCATTTCAGCCTTCCCCTGCGCCACAATTATCAGTACTCAGGCAGAAAAACT
GGTGGCTATGCTGCGCCGTACTGCAGCAGTTGCGCGTAATATTGAACGCCAGGATGACGCTTCGCTGGTACGTCATATCT
ACGACACATATTGTATCGTGCAGGCAGGCAATGTTGATATGTGTTTGCTTACTGATTTTTTTAAGCAAACCATTGAGCAA
GATATCCAGCGTTATGGCAATCAGTATCCGCAGTTTTGTGTTTCACCAAATGATGAGCTAAAAATGGGGCTGGACGAACT
TGAGCATAACCCGCTTTATAAGACACGCTACCAGCAATTTGTTGCCCCAATGGTATATGGTGCTCGCAAGGTATCGTGGG
ATGAAGCGTATGCTTCCTTTCGTCAAATGGCGTTAATAATTCTAGATACTTACTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT238650 WP_004181895.1 NZ_CP093459:c176608-176165 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT238650 NZ_LR890424:4624846-4625241 [Klebsiella pneumoniae]
ATGACGATAAAAACACGCATTCAGTCACGACTGAAACGCTCAAAACGCTATGTATTCACTCGTGACGATTTCAAGGATAT
TGCAGGCTATGATCAGATTGGCAGGGTATTGCGCGACCTTCTCAAAGAGGGGCAACTACTGAAGGTGGGCTACGGTGTGT
ACACCAAAGCGCGTCGGAACAGCATTACTGGAAAAGTCATGCCCGCAGCACCAAGGGGATCTTCGGCAGTGATCGTTGAA
ACGCTGGAGCGCCTGAAAGTACGTTACCGCTATGAAGGAGCTACACAGACATATAATAGTGGCGAATCCACGCAAATCCC
CGTGTCACCGGAGATCAAAACATCTCCGCGCTTTAAACGCGCGTTGTCTGTGGGGAACAGCAAACTAAATGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N4Z2R0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References