Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 37074..37717 Replicon plasmid pVA833-45
Accession NZ_CP093452
Organism Klebsiella pneumoniae strain VA833

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag MO408_RS27110 Protein ID WP_000754566.1
Coordinates 37301..37717 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag MO408_RS27105 Protein ID WP_001261276.1
Coordinates 37074..37304 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO408_RS27075 (MO408_27050) 32640..33506 - 867 WP_004118283.1 replication initiation protein -
MO408_RS27080 (MO408_27055) 34036..34140 + 105 WP_032409716.1 hypothetical protein -
MO408_RS27085 (MO408_27060) 34269..34526 + 258 WP_000764642.1 hypothetical protein -
MO408_RS27090 (MO408_27065) 34584..35359 - 776 Protein_42 site-specific integrase -
MO408_RS27095 (MO408_27070) 35356..36099 - 744 WP_000129823.1 hypothetical protein -
MO408_RS27100 (MO408_27075) 36150..36500 - 351 WP_000493378.1 hypothetical protein -
MO408_RS27105 (MO408_27080) 37074..37304 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MO408_RS27110 (MO408_27085) 37301..37717 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
MO408_RS27115 (MO408_27090) 37921..38777 + 857 Protein_47 IS3-like element ISEc15 family transposase -
MO408_RS27120 (MO408_27095) 39059..39259 + 201 Protein_48 transposase -
MO408_RS27125 (MO408_27100) 39282..39704 + 423 WP_032446862.1 hypothetical protein -
MO408_RS27130 (MO408_27105) 39713..40210 + 498 WP_032446861.1 hypothetical protein -
MO408_RS27135 (MO408_27110) 40347..40682 - 336 Protein_51 Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid qacE / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 - 1..44806 44806


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T238631 WP_000754566.1 NZ_CP093452:37301-37717 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T238631 NZ_LR890417:58597-59079 [Klebsiella pneumoniae]
GTGGGACGTTTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTGGCTGAGTTCGTCAGCGGTGAGGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCCCTCGGAGCTGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGCAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGCGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAGCGAACATTGTTTCTTAGACTCCCT
TAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT238631 WP_001261276.1 NZ_CP093452:37074-37304 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT238631 NZ_LR890417:58322-58609 [Klebsiella pneumoniae]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAATGGCCTGTCAGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGCGGAGTTCATCGACATGCTTGATGCACCTGTCGTAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References