Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1967619..1968450 Replicon chromosome
Accession NC_012759
Organism Escherichia coli BW2952

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag BWG_RS09935 Protein ID WP_000854814.1
Coordinates 1968076..1968450 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag BWG_RS09930 Protein ID WP_001285584.1
Coordinates 1967619..1967987 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWG_RS09915 (BWG_1792) 1965286..1966818 + 1533 WP_001350525.1 hypothetical protein -
BWG_RS25050 (BWG_1793) 1966815..1967261 + 447 WP_000187523.1 RadC family protein -
BWG_RS09925 (BWG_1794) 1967324..1967545 + 222 WP_000692323.1 DUF987 domain-containing protein -
BWG_RS09930 (BWG_1795) 1967619..1967987 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
BWG_RS09935 (BWG_1796) 1968076..1968450 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
BWG_RS09940 1968447..1968641 + 195 WP_000988600.1 hypothetical protein -
BWG_RS25565 1968654..1968767 + 114 WP_001161660.1 DUF957 domain-containing protein -
BWG_RS23825 1969056..1969184 - 129 Protein_1898 transposase domain-containing protein -
BWG_RS09950 1969256..1969438 + 183 WP_001016348.1 hypothetical protein -
BWG_RS09955 (BWG_1797) 1969539..1969868 - 330 WP_000450409.1 DUF496 family protein -
BWG_RS09960 (BWG_1798) 1970040..1971098 - 1059 WP_001200891.1 FUSC family protein -
BWG_RS09965 (BWG_1799) 1971296..1971769 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
BWG_RS09970 (BWG_1800) 1971888..1973054 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T23863 WP_000854814.1 NC_012759:1968076-1968450 [Escherichia coli BW2952]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T23863 NC_012759:1968076-1968450 [Escherichia coli BW2952]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT23863 WP_001285584.1 NC_012759:1967619-1967987 [Escherichia coli BW2952]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT23863 NC_012759:1967619-1967987 [Escherichia coli BW2952]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References