Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5380005..5380630 Replicon chromosome
Accession NZ_CP093451
Organism Klebsiella pneumoniae strain VA833

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MO408_RS26515 Protein ID WP_002882817.1
Coordinates 5380005..5380388 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MO408_RS26520 Protein ID WP_004150355.1
Coordinates 5380388..5380630 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO408_RS26500 (5377371) 5377371..5378273 + 903 WP_004901686.1 formate dehydrogenase subunit beta -
MO408_RS26505 (5378270) 5378270..5378905 + 636 WP_004901683.1 formate dehydrogenase cytochrome b556 subunit -
MO408_RS26510 (5378902) 5378902..5379831 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MO408_RS26515 (5380005) 5380005..5380388 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MO408_RS26520 (5380388) 5380388..5380630 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MO408_RS26525 (5380835) 5380835..5381752 + 918 WP_004901674.1 alpha/beta hydrolase -
MO408_RS26530 (5381766) 5381766..5382707 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MO408_RS26535 (5382752) 5382752..5383189 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MO408_RS26540 (5383186) 5383186..5384046 - 861 WP_002882807.1 virulence factor BrkB family protein -
MO408_RS26545 (5384040) 5384040..5384639 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T238629 WP_002882817.1 NZ_CP093451:c5380388-5380005 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T238629 NZ_LR890417:c20853-20437 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT238629 WP_004150355.1 NZ_CP093451:c5380630-5380388 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT238629 NZ_LR890417:c21080-20850 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References