Detailed information of TA system TA238585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1822764..1823803
• Replicon: chromosome
• Accession: NZ_CP093428
• Organism: Pseudomonas migulae strain 8R6

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: MOQ58_RS08345
• Protein ID: WP_280163304.1
• Coordinates: 1822764..1823339 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: MOQ58_RS08350
• Protein ID: WP_280163305.1
• Coordinates: 1823336..1823803 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ58_RS08320 (MOQ58_08305)	1818102..1820090	+	1989	WP_280163303.1	heme lyase CcmF/NrfE family subunit	-
MOQ58_RS08325 (MOQ58_08310)	1820087..1820623	+	537	WP_226298828.1	DsbE family thiol:disulfide interchange protein	-
MOQ58_RS08330 (MOQ58_08315)	1820620..1821093	+	474	WP_192419640.1	cytochrome c-type biogenesis protein CcmH	-
MOQ58_RS08335 (MOQ58_08320)	1821086..1822288	+	1203	WP_008039841.1	c-type cytochrome biogenesis protein CcmI	-
MOQ58_RS08340 (MOQ58_08325)	1822303..1822707	+	405	WP_008026806.1	hypothetical protein	-
MOQ58_RS08345 (MOQ58_08330)	1822764..1823339	-	576	WP_280163304.1	PIN domain-containing protein	Toxin
MOQ58_RS08350 (MOQ58_08335)	1823336..1823803	-	468	WP_280163305.1	helix-turn-helix domain-containing protein	Antitoxin
MOQ58_RS08355 (MOQ58_08340)	1823885..1824154	-	270	Protein_1645	LysR substrate-binding domain-containing protein	-
MOQ58_RS08360 (MOQ58_08345)	1824194..1824913	-	720	WP_008026798.1	SDR family oxidoreductase	-
MOQ58_RS08365 (MOQ58_08350)	1825195..1825407	+	213	WP_280163306.1	type II toxin-antitoxin system HicA family toxin	-
MOQ58_RS08370 (MOQ58_08355)	1825407..1825811	+	405	WP_192226071.1	type II toxin-antitoxin system HicB family antitoxin	-
MOQ58_RS08375 (MOQ58_08360)	1825924..1827129	-	1206	WP_280163307.1	MFS transporter	-
MOQ58_RS08380 (MOQ58_08365)	1827344..1827961	+	618	WP_253402296.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21655.81 Da
• Isoelectric Point: 5.0417

>T238585 WP_280163304.1 NZ_CP093428:c1823339-1822764 [Pseudomonas migulae]
VRHSSFTAVYDACVLYPAPLRDFLMWLALSGRFRAKWTMEIHNEWKRNLLKNRPDLTTGQLNRTSELMDQALPDACVSGY
EELIEGLTLPDVDDRHVLAVAIHCNASVIVTFNQKDFPCSALSPFGIEAQHPDEFVDNLFDLDPAAVIEAAQRQRKQLKL
PPMDVDSYLELLLRQGLVQSSKALTEYRAIL

Nucleotide

• Length: 576 bp

>T238585 NZ_LR890405:4293144-4293296 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17301.75 Da
• Isoelectric Point: 5.6017

>AT238585 WP_280163305.1 NZ_CP093428:c1823803-1823336 [Pseudomonas migulae]
MSPTLLPTINLPLEREVQAAIEGQRALAAYLATQFETQRIQIFDEQNEAHSVELPTSALRLLVDILEALAAGNAVKVVPV
HAELTTQEAADLLNVSRPHLIKLLESGDLSYHKTGKHRRVRFADLMDYKNRRDTASEQAMMLLAEQAQALRTGYE

Nucleotide

• Length: 468 bp

>AT238585 NZ_LR890405:c4293095-4293038 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA