Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1535121..1535745 Replicon chromosome
Accession NZ_CP093428
Organism Pseudomonas migulae strain 8R6

Toxin (Protein)


Gene name hicA Uniprot ID J3GAV2
Locus tag MOQ58_RS06945 Protein ID WP_007984690.1
Coordinates 1535566..1535745 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID J3BXS4
Locus tag MOQ58_RS06940 Protein ID WP_008036180.1
Coordinates 1535121..1535528 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOQ58_RS06905 (MOQ58_06900) 1530360..1530518 + 159 WP_008025734.1 KGG domain-containing protein -
MOQ58_RS06910 (MOQ58_06905) 1530592..1531095 + 504 WP_192420576.1 DUF4142 domain-containing protein -
MOQ58_RS06915 (MOQ58_06910) 1531185..1531595 - 411 WP_280163185.1 low affinity iron permease family protein -
MOQ58_RS06920 (MOQ58_06915) 1531780..1532316 - 537 WP_280163186.1 AidA/PixA family protein -
MOQ58_RS06925 (MOQ58_06920) 1532443..1532946 - 504 WP_280163187.1 inclusion body family protein -
MOQ58_RS06930 (MOQ58_06925) 1533340..1534110 + 771 WP_280163188.1 trans-aconitate 2-methyltransferase -
MOQ58_RS06935 (MOQ58_06930) 1534416..1534682 - 267 WP_192228676.1 short-chain dehydrogenase -
MOQ58_RS06940 (MOQ58_06935) 1535121..1535528 - 408 WP_008036180.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MOQ58_RS06945 (MOQ58_06940) 1535566..1535745 - 180 WP_007984690.1 type II toxin-antitoxin system HicA family toxin Toxin
MOQ58_RS06950 (MOQ58_06945) 1535997..1537541 - 1545 WP_280163189.1 Fic family protein -
MOQ58_RS06955 (MOQ58_06950) 1537868..1538206 - 339 WP_192419439.1 hypothetical protein -
MOQ58_RS06960 (MOQ58_06955) 1538661..1540139 + 1479 WP_280163190.1 cytochrome c oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6704.97 Da        Isoelectric Point: 11.4113

>T238584 WP_007984690.1 NZ_CP093428:c1535745-1535566 [Pseudomonas migulae]
VNSRFLISQIVADGWYLVRIRGSHHHFKHPTKPGLVTVPHPKKDLLKKTAISILQQALL

Download         Length: 180 bp

>T238584 NZ_LR890405:c3995382-3995041 [Escherichia coli]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTACTTGC
TGCAATGCTGATTCTTGCTGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGATACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGCGTCCAACATCAGGGAAACCCGGTACGTGCTTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCGGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAAGCATCTGAAAAAGTAA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14662.64 Da        Isoelectric Point: 4.3833

>AT238584 WP_008036180.1 NZ_CP093428:c1535528-1535121 [Pseudomonas migulae]
MLYPIAISMGDDEHAWGVEVPDIPGCFSAGEDLDDAMAMAREAIEGHFEILAEDGSPIPPANKVTLHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQS

Download         Length: 408 bp

>AT238584 NZ_LR890405:c3995031-3994732 [Escherichia coli]
ATGGCTACTCTAAAAGAACTGATGGCAAAGCAAAGCCCTGAAAGCCAGGTGCGTATTGCGGCAAAAGTTGAGGAAATGCG
CCAGACCATCGCCTTGCATCAGCTGAGAGAAGAACTCAATATTTCGCAAACGGAAATGGCGGCAGCGATGGGCATTAAGC
AACCGACTCTTGCAAAAATGGAGCAGGCAGATAACGATCCTCGGTTGTCTACGCTAAAACGCTATGTTGCGGCTTTAGGT
GGGGAATTGAGTATCGACGTTAAACTGCCGAATGGTAAGAGAATTGCATTTCAGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J3GAV2

Antitoxin

Source ID Structure
AlphaFold DB A0A291ADB7

References