Detailed information of TA system TA023852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1399369..1400007
• Replicon: chromosome
• Accession: NC_012759
• Organism: Escherichia coli BW2952

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: BWG_RS07005
• Protein ID: WP_000813794.1
• Coordinates: 1399369..1399545 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BWG_RS07010
• Protein ID: WP_001270286.1
• Coordinates: 1399591..1400007 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWG_RS06985 (BWG_1258)	1394988..1396202	-	1215	WP_001326689.1	BenE family transporter YdcO	-
BWG_RS06990 (BWG_1259)	1396255..1396791	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
BWG_RS06995 (BWG_1260)	1396864..1398825	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
BWG_RS07000 (BWG_1261)	1398917..1399147	-	231	WP_000494244.1	YncJ family protein	-
BWG_RS07005 (BWG_1262)	1399369..1399545	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
BWG_RS07010 (BWG_1263)	1399591..1400007	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
BWG_RS07015 (BWG_1264)	1400086..1401492	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
BWG_RS07020 (BWG_1265)	1401737..1402882	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
BWG_RS07025 (BWG_1266)	1402900..1403913	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
BWG_RS07030 (BWG_1267)	1403914..1404855	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T23852 WP_000813794.1 NC_012759:1399369-1399545 [Escherichia coli BW2952]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T23852 NC_012759:1399369-1399545 [Escherichia coli BW2952]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT23852 WP_001270286.1 NC_012759:1399591-1400007 [Escherichia coli BW2952]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT23852 NC_012759:1399591-1400007 [Escherichia coli BW2952]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB