Detailed information of TA system TA238440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 4450446..4450951
• Replicon: chromosome
• Accession: NZ_CP093395
• Organism: Pseudomonas aeruginosa strain PA1_NCHU

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: MON07_RS20845
• Protein ID: WP_003083773.1
• Coordinates: 4450670..4450951 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: MON07_RS20840
• Protein ID: WP_003083775.1
• Coordinates: 4450446..4450673 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MON07_RS20815 (MON07_20815)	4445465..4446958	+	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-
MON07_RS20820 (MON07_20820)	4447127..4448554	+	1428	WP_003083784.1	GABA permease	-
MON07_RS20825 (MON07_20825)	4448636..4448977	-	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
MON07_RS20830 (MON07_20830)	4449050..4449550	-	501	WP_003101228.1	LEA type 2 family protein	-
MON07_RS20835 (MON07_20835)	4449651..4450271	+	621	WP_003101226.1	hypothetical protein	-
MON07_RS20840 (MON07_20840)	4450446..4450673	+	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
MON07_RS20845 (MON07_20845)	4450670..4450951	+	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
MON07_RS20850 (MON07_20850)	4451251..4452159	+	909	WP_003083769.1	LysR family transcriptional regulator	-
MON07_RS20855 (MON07_20855)	4452191..4452601	-	411	WP_003101225.1	aegerolysin family protein	-
MON07_RS20860 (MON07_20860)	4452781..4453515	-	735	WP_003117208.1	GntR family transcriptional regulator	-
MON07_RS20865 (MON07_20865)	4453616..4454302	-	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
MON07_RS20870 (MON07_20870)	4454351..4455700	-	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T238440 WP_003083773.1 NZ_CP093395:4450670-4450951 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T238440 NZ_LR890343:4766720-4767130 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT238440 WP_003083775.1 NZ_CP093395:4450446-4450673 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT238440 NZ_LR890343:4766473-4766739 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9