Detailed information of TA system TA238347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 497534..498504
• Replicon: plasmid pLPS8
• Accession: NZ_CP093381
• Organism: Rhodococcus opacus strain S8

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: MOO23_RS38495
• Protein ID: WP_061041367.1
• Coordinates: 497534..498124 (-)
• Length: 197 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: MOO23_RS38500
• Protein ID: WP_061041368.1
• Coordinates: 498121..498504 (-)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOO23_RS38470 (MOO23_38470)	493338..493778	-	441	WP_242322870.1	DUF6262 family protein	-
MOO23_RS38475 (MOO23_38475)	493775..494626	-	852	WP_242323885.1	tyrosine-type recombinase/integrase	-
MOO23_RS38480 (MOO23_38480)	494508..495458	-	951	WP_242324294.1	hypothetical protein	-
MOO23_RS38485 (MOO23_38485)	495942..497159	-	1218	WP_242323887.1	tyrosine-type recombinase/integrase	-
MOO23_RS38490 (MOO23_38490)	497265..497476	-	212	Protein_467	DUF4193 family protein	-
MOO23_RS38495 (MOO23_38495)	497534..498124	-	591	WP_061041367.1	zeta toxin family protein	Toxin
MOO23_RS38500 (MOO23_38500)	498121..498504	-	384	WP_061041368.1	hypothetical protein	Antitoxin
MOO23_RS38505 (MOO23_38505)	499163..500167	-	1005	WP_242323889.1	acyl-CoA dehydrogenase family protein	-
MOO23_RS38510 (MOO23_38510)	500425..501735	-	1311	WP_242323891.1	Rep protein	-
MOO23_RS38515 (MOO23_38515)	502112..502429	-	318	WP_061044041.1	hypothetical protein	-
MOO23_RS38520 (MOO23_38520)	502426..502920	-	495	WP_242323893.1	hypothetical protein	-
MOO23_RS38525 (MOO23_38525)	502917..503162	-	246	WP_242323895.1	hypothetical protein	-
MOO23_RS38530 (MOO23_38530)	503228..503446	-	219	WP_242323897.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..788074	788074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (5-157)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (7-106)

Sequences

Toxin

Protein

• Length: 197 a.a.
• Molecular weight: 21625.50 Da
• Isoelectric Point: 7.0250

>T238347 WP_061041367.1 NZ_CP093381:c498124-497534 [Rhodococcus opacus]
VKRLDLVVGCNGAGKSTFVAFTLAPLLPASPFVNADEIATQRWPADPAAHSYEAAEIAAKTRAHLLTLGKSFIAETVFSH
PSKLELIDEARTHDYTVVLHVVMIPEELAVQRVRYRVRAGGHDVPEDKIRQRYHRLWGLVAQAFPRCDHVTVYDNSASTG
PRIVAQFTDGHLVGEATWPAWTPAELTSHTSRGEQP

Nucleotide

• Length: 591 bp

>T238347 NZ_LR890317:c4543906-4543373 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 13650.16 Da
• Isoelectric Point: 4.9627

>AT238347 WP_061041368.1 NZ_CP093381:c498504-498121 [Rhodococcus opacus]
MHSTGDKVTRLAADLVASAMAEGERQHRTGRQQLEHWARVGRAVSSRQTAARRRVEDALAGRLPIGELGPEEGTVFNAEI
NAAIEEQLATTDYGAALAAQGITTVALSEDGQIIEYRPDGTRTVLPE

Nucleotide

• Length: 384 bp

>AT238347 NZ_LR890317:c4544183-4543917 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
GGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA