Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 3983162..3983963 | Replicon | chromosome |
| Accession | NZ_CP093369 | ||
| Organism | Escherichia coli strain EV36 mutant 5a | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | D8EBK0 |
| Locus tag | L0Q35_RS19260 | Protein ID | WP_001094436.1 |
| Coordinates | 3983586..3983963 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | B7MN76 |
| Locus tag | L0Q35_RS19255 | Protein ID | WP_015953067.1 |
| Coordinates | 3983162..3983539 (+) | Length | 126 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L0Q35_RS19220 (3979074) | 3979074..3979754 | + | 681 | WP_001282927.1 | WYL domain-containing protein | - |
| L0Q35_RS19225 (3979902) | 3979902..3980579 | + | 678 | WP_001097312.1 | hypothetical protein | - |
| L0Q35_RS19230 (3980585) | 3980585..3980818 | + | 234 | WP_001278287.1 | DUF905 family protein | - |
| L0Q35_RS19235 (3980908) | 3980908..3981726 | + | 819 | WP_001175142.1 | DUF932 domain-containing protein | - |
| L0Q35_RS19240 (3981817) | 3981817..3982302 | + | 486 | WP_000860054.1 | antirestriction protein | - |
| L0Q35_RS19245 (3982317) | 3982317..3982793 | + | 477 | WP_001186756.1 | RadC family protein | - |
| L0Q35_RS19250 (3982862) | 3982862..3983083 | + | 222 | WP_000692312.1 | DUF987 domain-containing protein | - |
| L0Q35_RS19255 (3983162) | 3983162..3983539 | + | 378 | WP_015953067.1 | type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA | Antitoxin |
| L0Q35_RS19260 (3983586) | 3983586..3983963 | + | 378 | WP_001094436.1 | TA system toxin CbtA family protein | Toxin |
| L0Q35_RS19265 (3983960) | 3983960..3984448 | + | 489 | WP_000761714.1 | DUF5983 family protein | - |
| L0Q35_RS19270 (3984460) | 3984460..3984657 | + | 198 | WP_000839254.1 | DUF957 domain-containing protein | - |
| L0Q35_RS19275 (3984742) | 3984742..3985587 | + | 846 | WP_001280513.1 | DUF4942 domain-containing protein | - |
| L0Q35_RS19280 (3985658) | 3985658..3987193 | + | 1536 | WP_000492897.1 | EAL domain-containing protein | - |
| L0Q35_RS19285 (3987590) | 3987590..3987760 | + | 171 | Protein_3787 | IS110 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | papG / papF / papE / papK / papJ / papD / papC / papH / papA / papB / papI / kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / neuE / neuC / neuA / neuB / neuD / kpsT / kpsM / gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC / rfaE | 3932438..4126098 | 193660 | |
| - | flank | IS/Tn | - | - | 3987656..3987760 | 104 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 13958.84 Da Isoelectric Point: 6.8603
>T238295 WP_001094436.1 NZ_CP093369:3983586-3983963 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ
Download Length: 378 bp
>T238295 NZ_LR890299:c2148087-2147689 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 126 a.a. Molecular weight: 13766.60 Da Isoelectric Point: 6.2021
>AT238295 WP_015953067.1 NZ_CP093369:3983162-3983539 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV
Download Length: 378 bp
>AT238295 NZ_LR890299:c2148383-2148090 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E2L1N0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Z3CIS0 |