Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2899053..2899884 Replicon chromosome
Accession NZ_CP093369
Organism Escherichia coli strain EV36 mutant 5a

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag L0Q35_RS14145 Protein ID WP_000854814.1
Coordinates 2899510..2899884 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag L0Q35_RS14140 Protein ID WP_001285584.1
Coordinates 2899053..2899421 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0Q35_RS14125 (2896720) 2896720..2898252 + 1533 WP_001350525.1 protein YeeR -
L0Q35_RS14130 (2898249) 2898249..2898695 + 447 WP_000187523.1 RadC family protein -
L0Q35_RS14135 (2898758) 2898758..2898979 + 222 WP_000692323.1 DUF987 domain-containing protein -
L0Q35_RS14140 (2899053) 2899053..2899421 + 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
L0Q35_RS14145 (2899510) 2899510..2899884 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
L0Q35_RS14150 (2899881) 2899881..2900075 + 195 WP_000988600.1 DUF5983 family protein -
L0Q35_RS14155 (2900121) 2900121..2900201 + 81 Protein_2780 hypothetical protein -
L0Q35_RS14160 (2900490) 2900490..2900618 - 129 Protein_2781 transposase domain-containing protein -
L0Q35_RS14165 (2900738) 2900738..2900872 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
L0Q35_RS14170 (2900973) 2900973..2901302 - 330 WP_000450409.1 DUF496 family protein -
L0Q35_RS14175 (2901474) 2901474..2902532 - 1059 WP_001200891.1 FUSC family protein -
L0Q35_RS14180 (2902730) 2902730..2903203 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
L0Q35_RS14185 (2903322) 2903322..2904488 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T238289 WP_000854814.1 NZ_CP093369:2899510-2899884 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T238289 NZ_LR890299:1032888-1032995 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT238289 WP_001285584.1 NZ_CP093369:2899053-2899421 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT238289 NZ_LR890299:c1032841-1032775 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References