Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2331556..2332194 Replicon chromosome
Accession NZ_CP093369
Organism Escherichia coli strain EV36 mutant 5a

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag L0Q35_RS11255 Protein ID WP_000813794.1
Coordinates 2331556..2331732 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag L0Q35_RS11260 Protein ID WP_001270286.1
Coordinates 2331778..2332194 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0Q35_RS11235 (2327175) 2327175..2328350 - 1176 WP_001236265.1 BenE family transporter YdcO -
L0Q35_RS11240 (2328442) 2328442..2328978 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
L0Q35_RS11245 (2329051) 2329051..2331012 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
L0Q35_RS11250 (2331104) 2331104..2331334 - 231 WP_000494244.1 YncJ family protein -
L0Q35_RS11255 (2331556) 2331556..2331732 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
L0Q35_RS11260 (2331778) 2331778..2332194 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
L0Q35_RS11265 (2332273) 2332273..2333679 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
L0Q35_RS11270 (2333924) 2333924..2335069 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
L0Q35_RS11275 (2335087) 2335087..2336100 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
L0Q35_RS11280 (2336101) 2336101..2337042 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T238278 WP_000813794.1 NZ_CP093369:2331556-2331732 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T238278 NZ_LR890291:3976811-3977029 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT238278 WP_001270286.1 NZ_CP093369:2331778-2332194 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT238278 NZ_LR890291:3976410-3976784 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References