Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1338970..1339588 Replicon chromosome
Accession NZ_CP093369
Organism Escherichia coli strain EV36 mutant 5a

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag L0Q35_RS06445 Protein ID WP_001291435.1
Coordinates 1338970..1339188 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag L0Q35_RS06450 Protein ID WP_000344800.1
Coordinates 1339214..1339588 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0Q35_RS06410 (1334259) 1334259..1334831 + 573 WP_000779842.1 YbaY family lipoprotein -
L0Q35_RS06415 (1334862) 1334862..1335173 - 312 WP_000409911.1 MGMT family protein -
L0Q35_RS06425 (1335552) 1335552..1335905 + 354 WP_000878140.1 DUF1428 domain-containing protein -
L0Q35_RS06430 (1335947) 1335947..1337497 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
L0Q35_RS06435 (1337661) 1337661..1338131 - 471 WP_000136192.1 YlaC family protein -
L0Q35_RS06440 (1338247) 1338247..1338798 - 552 WP_000102564.1 maltose O-acetyltransferase -
L0Q35_RS06445 (1338970) 1338970..1339188 - 219 WP_001291435.1 HHA domain-containing protein Toxin
L0Q35_RS06450 (1339214) 1339214..1339588 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
L0Q35_RS06455 (1340134) 1340134..1343283 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
L0Q35_RS06460 (1343306) 1343306..1344499 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T238277 WP_001291435.1 NZ_CP093369:c1339188-1338970 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T238277 NZ_LR890291:c3804212-3803895 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT238277 WP_000344800.1 NZ_CP093369:c1339588-1339214 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT238277 NZ_LR890291:c3803902-3803615 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References