Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 602946..603443 Replicon chromosome
Accession NZ_CP093369
Organism Escherichia coli strain EV36 mutant 5a

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag L0Q35_RS02865 Protein ID WP_001173343.1
Coordinates 603270..603443 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag L0Q35_RS02860 Protein ID WP_000398619.1
Coordinates 602946..603242 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0Q35_RS02840 (599677) 599677..600396 - 720 WP_000611288.1 two-component system response regulator DcuR -
L0Q35_RS02845 (600393) 600393..602024 - 1632 WP_001216477.1 sensor histidine kinase -
L0Q35_RS02850 (602205) 602205..602435 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
L0Q35_RS02855 (602447) 602447..602719 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
L0Q35_RS02860 (602946) 602946..603242 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
L0Q35_RS02865 (603270) 603270..603443 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
L0Q35_RS02870 (603562) 603562..605079 - 1518 WP_001295074.1 lysine--tRNA ligase -
L0Q35_RS02875 (605316) 605316..606773 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T238270 WP_001173343.1 NZ_CP093369:603270-603443 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T238270 NZ_LR890290:18408-18566 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT238270 WP_000398619.1 NZ_CP093369:602946-603242 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT238270 NZ_LR890290:18297-18362 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References