Detailed information of TA system TA238266

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4015520..4016321
• Replicon: chromosome
• Accession: NZ_CP093368
• Organism: Escherichia coli strain EV36 mutant 1a

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D8EBK0
• Locus tag: L0S95_RS19465
• Protein ID: WP_001094436.1
• Coordinates: 4015944..4016321 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MN76
• Locus tag: L0S95_RS19460
• Protein ID: WP_015953067.1
• Coordinates: 4015520..4015897 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0S95_RS19425 (4011432)	4011432..4012112	+	681	WP_001282927.1	WYL domain-containing protein	-
L0S95_RS19430 (4012260)	4012260..4012937	+	678	WP_001097312.1	hypothetical protein	-
L0S95_RS19435 (4012943)	4012943..4013176	+	234	WP_001278287.1	DUF905 family protein	-
L0S95_RS19440 (4013266)	4013266..4014084	+	819	WP_001175142.1	DUF932 domain-containing protein	-
L0S95_RS19445 (4014175)	4014175..4014660	+	486	WP_000860054.1	antirestriction protein	-
L0S95_RS19450 (4014675)	4014675..4015151	+	477	WP_001186756.1	RadC family protein	-
L0S95_RS19455 (4015220)	4015220..4015441	+	222	WP_000692312.1	DUF987 domain-containing protein	-
L0S95_RS19460 (4015520)	4015520..4015897	+	378	WP_015953067.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
L0S95_RS19465 (4015944)	4015944..4016321	+	378	WP_001094436.1	TA system toxin CbtA family protein	Toxin
L0S95_RS19470 (4016318)	4016318..4016806	+	489	WP_000761714.1	DUF5983 family protein	-
L0S95_RS19475 (4016818)	4016818..4017015	+	198	WP_000839254.1	DUF957 domain-containing protein	-
L0S95_RS19480 (4017100)	4017100..4017945	+	846	WP_001280513.1	DUF4942 domain-containing protein	-
L0S95_RS19485 (4018016)	4018016..4019551	+	1536	WP_000492897.1	EAL domain-containing protein	-
L0S95_RS19490 (4019948)	4019948..4020118	+	171	Protein_3826	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	papG / papF / papE / papK / papJ / papD / papC / papH / papA / papB / papI / kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / neuE / neuC / neuA / neuB / neuD / kpsT / kpsM / gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC / rfaE	3964796..4157120	192324
	-	flank	IS/Tn	-	-	4020014..4020118	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13958.84 Da
• Isoelectric Point: 6.8603

>T238266 WP_001094436.1 NZ_CP093368:4015944-4016321 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T238266 NZ_LR890289:c130586-130428 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13766.60 Da
• Isoelectric Point: 6.2021

>AT238266 WP_015953067.1 NZ_CP093368:4015520-4015897 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT238266 NZ_LR890289:c130661-130630 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: A0A0E2L1N0

Antitoxin

• AlphaFold DB: A0A5Z3CIS0