Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3895012..3895666 Replicon chromosome
Accession NZ_CP093368
Organism Escherichia coli strain EV36 mutant 1a

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag L0S95_RS18825 Protein ID WP_000244781.1
Coordinates 3895012..3895419 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L0S95_RS18830 Protein ID WP_000354046.1
Coordinates 3895400..3895666 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0S95_RS18805 (3890969) 3890969..3892702 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
L0S95_RS18810 (3892708) 3892708..3893418 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L0S95_RS18815 (3893443) 3893443..3894339 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
L0S95_RS18820 (3894451) 3894451..3894972 + 522 WP_001055874.1 flavodoxin FldB -
L0S95_RS18825 (3895012) 3895012..3895419 - 408 WP_000244781.1 protein YgfX Toxin
L0S95_RS18830 (3895400) 3895400..3895666 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L0S95_RS18835 (3895909) 3895909..3896889 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
L0S95_RS18840 (3896966) 3896966..3897625 - 660 WP_000250274.1 hemolysin III family protein -
L0S95_RS18845 (3897789) 3897789..3898100 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
L0S95_RS18850 (3898145) 3898145..3899578 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T238265 WP_000244781.1 NZ_CP093368:c3895419-3895012 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T238265 NZ_LR890289:c85518-85312 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT238265 WP_000354046.1 NZ_CP093368:c3895666-3895400 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT238265 NZ_LR890289:85505-85566 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References