Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2363913..2364551 Replicon chromosome
Accession NZ_CP093368
Organism Escherichia coli strain EV36 mutant 1a

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag L0S95_RS11460 Protein ID WP_000813794.1
Coordinates 2363913..2364089 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag L0S95_RS11465 Protein ID WP_001270286.1
Coordinates 2364135..2364551 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0S95_RS11440 (2359532) 2359532..2360707 - 1176 WP_001236265.1 BenE family transporter YdcO -
L0S95_RS11445 (2360799) 2360799..2361335 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
L0S95_RS11450 (2361408) 2361408..2363369 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
L0S95_RS11455 (2363461) 2363461..2363691 - 231 WP_000494244.1 YncJ family protein -
L0S95_RS11460 (2363913) 2363913..2364089 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
L0S95_RS11465 (2364135) 2364135..2364551 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
L0S95_RS11470 (2364630) 2364630..2366036 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
L0S95_RS11475 (2366281) 2366281..2367426 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
L0S95_RS11480 (2367444) 2367444..2368457 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
L0S95_RS11485 (2368458) 2368458..2369399 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T238249 WP_000813794.1 NZ_CP093368:2363913-2364089 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T238249 NZ_LR890288:1829495-1829746 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT238249 WP_001270286.1 NZ_CP093368:2364135-2364551 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT238249 NZ_LR890288:1829743-1829997 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References