Detailed information of TA system TA238249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2363913..2364551
• Replicon: chromosome
• Accession: NZ_CP093368
• Organism: Escherichia coli strain EV36 mutant 1a

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: L0S95_RS11460
• Protein ID: WP_000813794.1
• Coordinates: 2363913..2364089 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: L0S95_RS11465
• Protein ID: WP_001270286.1
• Coordinates: 2364135..2364551 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0S95_RS11440 (2359532)	2359532..2360707	-	1176	WP_001236265.1	BenE family transporter YdcO	-
L0S95_RS11445 (2360799)	2360799..2361335	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
L0S95_RS11450 (2361408)	2361408..2363369	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
L0S95_RS11455 (2363461)	2363461..2363691	-	231	WP_000494244.1	YncJ family protein	-
L0S95_RS11460 (2363913)	2363913..2364089	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
L0S95_RS11465 (2364135)	2364135..2364551	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
L0S95_RS11470 (2364630)	2364630..2366036	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
L0S95_RS11475 (2366281)	2366281..2367426	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
L0S95_RS11480 (2367444)	2367444..2368457	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
L0S95_RS11485 (2368458)	2368458..2369399	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T238249 WP_000813794.1 NZ_CP093368:2363913-2364089 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T238249 NZ_LR890288:1829495-1829746 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT238249 WP_001270286.1 NZ_CP093368:2364135-2364551 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT238249 NZ_LR890288:1829743-1829997 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• PDB: 6HPB