Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 1154565..1155244 | Replicon | chromosome |
| Accession | NZ_CP093368 | ||
| Organism | Escherichia coli strain EV36 mutant 1a | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | P77692 |
| Locus tag | L0S95_RS05540 | Protein ID | WP_000854672.1 |
| Coordinates | 1154565..1154906 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | Q47684 |
| Locus tag | L0S95_RS05545 | Protein ID | WP_000070395.1 |
| Coordinates | 1154927..1155244 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L0S95_RS05515 (1149842) | 1149842..1150243 | + | 402 | WP_000174689.1 | sigma factor-binding protein Crl | - |
| L0S95_RS05520 (1150282) | 1150282..1151337 | - | 1056 | WP_000749863.1 | phosphoporin PhoE | - |
| L0S95_RS05525 (1151625) | 1151625..1152728 | + | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| L0S95_RS05530 (1152740) | 1152740..1153993 | + | 1254 | WP_000893278.1 | glutamate-5-semialdehyde dehydrogenase | - |
| L0S95_RS05540 (1154565) | 1154565..1154906 | - | 342 | WP_000854672.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| L0S95_RS05545 (1154927) | 1154927..1155244 | - | 318 | WP_000070395.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| L0S95_RS05550 (1155263) | 1155263..1155484 | - | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| L0S95_RS05555 (1155493) | 1155493..1155969 | - | 477 | WP_000811693.1 | RadC family protein | - |
| L0S95_RS05560 (1155985) | 1155985..1156443 | - | 459 | WP_000211838.1 | antirestriction protein | - |
| L0S95_RS05565 (1156541) | 1156541..1156780 | - | 240 | WP_000194654.1 | DUF905 family protein | - |
| L0S95_RS05570 (1156857) | 1156857..1157324 | - | 468 | WP_001547765.1 | protein YkfB | - |
| L0S95_RS05575 (1157347) | 1157347..1157790 | - | 444 | WP_000824223.1 | lipoprotein YafY | - |
| L0S95_RS05580 (1157790) | 1157790..1158017 | - | 228 | WP_001548158.1 | protein YpjK | - |
| L0S95_RS05585 (1158013) | 1158013..1158204 | - | 192 | Protein_1097 | DeoR family transcriptional regulator | - |
| L0S95_RS05590 (1158421) | 1158421..1159242 | - | 822 | WP_000197389.1 | DUF932 domain-containing protein | - |
| L0S95_RS05595 (1159334) | 1159334..1160197 | - | 864 | WP_001065553.1 | GTPase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 1154345..1188258 | 33913 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12905.01 Da Isoelectric Point: 9.6543
>T238247 WP_000854672.1 NZ_CP093368:c1154906-1154565 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T238247 NZ_LR890288:992054-992389 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG
Antitoxin
Download Length: 106 a.a. Molecular weight: 11939.59 Da Isoelectric Point: 6.7391
>AT238247 WP_000070395.1 NZ_CP093368:c1155244-1154927 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT238247 NZ_LR890288:991806-992054 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|