Detailed information of TA system TA238237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 689313..689914
• Replicon: chromosome
• Accession: NZ_CP093365
• Organism: Bombilactobacillus thymidiniphilus strain SG4_A1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: MOO47_RS03725
• Protein ID: WP_249513454.1
• Coordinates: 689313..689657 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: MOO47_RS03730
• Protein ID: WP_249513455.1
• Coordinates: 689651..689914 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOO47_RS03685 (MOO47_03685)	684720..685115	-	396	WP_249513446.1	DUF1310 family protein	-
MOO47_RS03690 (MOO47_03690)	685196..685477	-	282	WP_249513447.1	hypothetical protein	-
MOO47_RS03695 (MOO47_03695)	685482..685868	-	387	WP_249513448.1	DUF1310 family protein	-
MOO47_RS03700 (MOO47_03700)	686038..686430	-	393	WP_249513449.1	DUF1310 family protein	-
MOO47_RS03705 (MOO47_03705)	686577..686966	-	390	WP_249513450.1	DUF1310 family protein	-
MOO47_RS03710 (MOO47_03710)	687149..687544	-	396	WP_249513451.1	DUF1310 family protein	-
MOO47_RS03715 (MOO47_03715)	687695..688093	-	399	WP_249513452.1	DUF1310 family protein	-
MOO47_RS03720 (MOO47_03720)	688475..689209	-	735	WP_249513453.1	choloylglycine hydrolase family protein	-
MOO47_RS03725 (MOO47_03725)	689313..689657	-	345	WP_249513454.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MOO47_RS03730 (MOO47_03730)	689651..689914	-	264	WP_249513455.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MOO47_RS03735 (MOO47_03735)	690009..690587	+	579	WP_249513536.1	site-specific integrase	-
MOO47_RS03740 (MOO47_03740)	690605..690793	+	189	WP_249513456.1	hypothetical protein	-
MOO47_RS03745 (MOO47_03745)	690926..692833	+	1908	WP_249513537.1	DNA methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-101)

Antitoxin

• Antitoxin PemI-like (8-84)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13001.91 Da
• Isoelectric Point: 6.7231

>T238237 WP_249513454.1 NZ_CP093365:c689657-689313 [Bombilactobacillus thymidiniphilus]
MVKQGDIFYVNFSPSRGHEQMYRRPAIALSHDMVAQTSNMTIVAPISSTTRDFPMYHQLTSSRNVYGKVLLDQTIALDLR
ARQVKNDDIVDQVTRAELVEIIDLYKLLFSIDPA

Nucleotide

• Length: 345 bp

>T238237 NZ_LR890286:3878708-3878926 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9879.15 Da
• Isoelectric Point: 5.0484

>AT238237 WP_249513455.1 NZ_CP093365:c689914-689651 [Bombilactobacillus thymidiniphilus]
MALIQAQTRLAKWGNSQAARIPSQIIKKLQLADNQELDVTVENGAIVLTPVRKQPANIHELFKDWHDDGIREHELDWGQA
TGDELPW

Nucleotide

• Length: 264 bp

>AT238237 NZ_LR890286:3878307-3878681 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG