Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 678329..679139 Replicon chromosome
Accession NC_012731
Organism Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag KP1_RS03330 Protein ID WP_014908042.1
Coordinates 678606..679139 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KP1_RS03325 Protein ID WP_002887278.1
Coordinates 678329..678595 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP1_RS03300 674201..674545 - 345 WP_012737331.1 cation efflux system protein CusF -
KP1_RS03305 674563..675948 - 1386 WP_012737332.1 efflux transporter outer membrane subunit -
KP1_RS03315 676120..676803 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
KP1_RS03320 676793..678226 + 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
KP1_RS03325 678329..678595 + 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
KP1_RS03330 678606..679139 + 534 WP_014908042.1 GNAT family N-acetyltransferase Toxin
KP1_RS03335 679187..680308 - 1122 WP_012737335.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T23823 WP_014908042.1 NC_012731:678606-679139 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T23823 NC_012731:678606-679139 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT23823 WP_002887278.1 NC_012731:678329-678595 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT23823 NC_012731:678329-678595 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References