Detailed information of TA system TA238224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2705868..2706910
• Replicon: chromosome
• Accession: NZ_CP093357
• Organism: Pseudomonas aeruginosa strain E131

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: MN179_RS12810
• Protein ID: WP_003153636.1
• Coordinates: 2706335..2706910 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: MN179_RS12805
• Protein ID: WP_003050245.1
• Coordinates: 2705868..2706338 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN179_RS12770 (MN179_12770)	2701260..2702678	-	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-
MN179_RS12775 (MN179_12775)	2702668..2703579	-	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
MN179_RS12780 (MN179_12780)	2703576..2704268	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
MN179_RS12785 (MN179_12785)	2704265..2704663	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
MN179_RS12790 (MN179_12790)	2704675..2705034	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
MN179_RS12795 (MN179_12795)	2705051..2705284	-	234	WP_003090170.1	TIGR03758 family integrating conjugative element protein	-
MN179_RS12800 (MN179_12800)	2705281..2705664	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
MN179_RS12805 (MN179_12805)	2705868..2706338	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
MN179_RS12810 (MN179_12810)	2706335..2706910	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
MN179_RS12815 (MN179_12815)	2706928..2707842	+	915	WP_023114497.1	AAA family ATPase	-
MN179_RS12820 (MN179_12820)	2707839..2708309	+	471	WP_023114496.1	type III CBASS phage resistance system CD-NTase-associated protein Cap8	-
MN179_RS12825 (MN179_12825)	2708306..2708806	+	501	WP_003090159.1	type III CBASS phage resistance system CD-NTase-associated protein Cap7	-
MN179_RS12830 (MN179_12830)	2708806..2709708	+	903	WP_003090158.1	CBASS oligonucleotide cyclase	-
MN179_RS12835 (MN179_12835)	2709747..2710472	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2631303..2753026	121723

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T238224 WP_003153636.1 NZ_CP093357:2706335-2706910 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T238224 NZ_LR890277:c4450036-4449503 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATATGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCTGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT238224 WP_003050245.1 NZ_CP093357:2705868-2706338 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT238224 NZ_LR890277:c4450313-4450047 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: I3TV68