Detailed information of TA system TA238222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1305694..1306364
• Replicon: chromosome
• Accession: NZ_CP093357
• Organism: Pseudomonas aeruginosa strain E131

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MN179_RS06105
• Protein ID: WP_023114647.1
• Coordinates: 1305945..1306364 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MN179_RS06100
• Protein ID: WP_023114648.1
• Coordinates: 1305694..1305948 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN179_RS06080 (MN179_06080)	1301235..1302227	-	993	WP_023114651.1	hypothetical protein	-
MN179_RS06085 (MN179_06085)	1302321..1302545	+	225	WP_023114650.1	AlpA family phage regulatory protein	-
MN179_RS06090 (MN179_06090)	1302548..1303441	+	894	WP_031637438.1	helicase RepA family protein	-
MN179_RS06095 (MN179_06095)	1303422..1304399	+	978	WP_031637437.1	replication protein C, IncQ-type	-
MN179_RS06100 (MN179_06100)	1305694..1305948	+	255	WP_023114648.1	Arc family DNA-binding protein	Antitoxin
MN179_RS06105 (MN179_06105)	1305945..1306364	+	420	WP_023114647.1	type II toxin-antitoxin system VapC family toxin	Toxin
MN179_RS06110 (MN179_06110)	1306520..1307290	+	771	WP_023114646.1	P-type conjugative transfer protein TrbJ	-
MN179_RS06115 (MN179_06115)	1307304..1307462	+	159	WP_023114645.1	hypothetical protein	-
MN179_RS06120 (MN179_06120)	1307464..1308936	+	1473	WP_023114644.1	P-type conjugative transfer protein TrbL	-
MN179_RS06125 (MN179_06125)	1309005..1309247	+	243	WP_023114643.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
MN179_RS06130 (MN179_06130)	1309259..1309543	+	285	WP_023114642.1	hypothetical protein	-
MN179_RS06135 (MN179_06135)	1309546..1309914	+	369	WP_023114641.1	conjugal transfer transcriptional regulator TraJ	-
MN179_RS06140 (MN179_06140)	1309953..1310183	-	231	WP_229033214.1	hypothetical protein	-
MN179_RS06145 (MN179_06145)	1310163..1310411	-	249	Protein_1217	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1301235..1320846	19611

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14925.16 Da
• Isoelectric Point: 5.1890

>T238222 WP_023114647.1 NZ_CP093357:1305945-1306364 [Pseudomonas aeruginosa]
MIVLDTNVVSEAMKPEPNPAVRAWLNEQVVETLYLSSVTLAELLFGIGALPNGKRKKGLGEALDGLLELFGERVLMFDTE
AARHYAELAVKARTAGKGFPTPDGYIGAIAASKGFIVATRDTSPFEAAGLTVINPWNHQ

Nucleotide

• Length: 420 bp

>T238222 NZ_LR890277:c3717468-3717151 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9508.87 Da
• Isoelectric Point: 6.4760

>AT238222 WP_023114648.1 NZ_CP093357:1305694-1305948 [Pseudomonas aeruginosa]
MAILTVRNVPDEVHRALRLRAAEHGRSTEAEVREILESAVKSEKRIRMGDALAELGRRVGLTNDDFAVLDQVRDKVPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT238222 NZ_LR890277:c3717158-3716871 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACGTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA