Detailed information of TA system TA238219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5568318..5568958
• Replicon: chromosome
• Accession: NZ_CP093356
• Organism: Pseudomonas aeruginosa strain E125

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MN178_RS26210
• Protein ID: WP_003105740.1
• Coordinates: 5568318..5568728 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6X2S2
• Locus tag: MN178_RS26215
• Protein ID: WP_003158175.1
• Coordinates: 5568728..5568958 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN178_RS26170 (MN178_26170)	5564187..5564357	+	171	WP_003159716.1	hypothetical protein	-
MN178_RS26175 (MN178_26175)	5564513..5565241	+	729	WP_003105754.1	TIGR03761 family integrating conjugative element protein	-
MN178_RS26180 (MN178_26180)	5565247..5565795	+	549	WP_003105753.1	DUF3158 family protein	-
MN178_RS26185 (MN178_26185)	5565842..5566681	+	840	WP_003105750.1	Rha family transcriptional regulator	-
MN178_RS26190 (MN178_26190)	5566711..5567199	+	489	WP_003105748.1	single-stranded DNA-binding protein	-
MN178_RS26195 (MN178_26195)	5567355..5567552	+	198	WP_003109353.1	CrpP family ICE-associated protein	-
MN178_RS26200 (MN178_26200)	5567618..5567836	-	219	WP_003105747.1	hypothetical protein	-
MN178_RS26205 (MN178_26205)	5568036..5568296	-	261	WP_003105742.1	hypothetical protein	-
MN178_RS26210 (MN178_26210)	5568318..5568728	-	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
MN178_RS26215 (MN178_26215)	5568728..5568958	-	231	WP_003158175.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MN178_RS26220 (MN178_26220)	5569214..5571133	+	1920	WP_004352838.1	type I DNA topoisomerase	-
MN178_RS26225 (MN178_26225)	5571441..5571650	+	210	WP_003105733.1	cold-shock protein	-
MN178_RS26230 (MN178_26230)	5571871..5573760	+	1890	WP_003105732.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5548747..5652324	103577

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T238219 WP_003105740.1 NZ_CP093356:c5568728-5568318 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T238219 NZ_LR890277:792832-793242 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.41 Da
• Isoelectric Point: 4.2821

>AT238219 WP_003158175.1 NZ_CP093356:c5568958-5568728 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVVVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT238219 NZ_LR890277:792585-792851 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: Q6X2S2