Detailed information of TA system TA238217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3841144..3842365
• Replicon: chromosome
• Accession: NZ_CP093356
• Organism: Pseudomonas aeruginosa strain E125

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: MN178_RS17945
• Protein ID: WP_003102740.1
• Coordinates: 3841787..3842365 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MN178_RS17940
• Protein ID: WP_003088112.1
• Coordinates: 3841144..3841698 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN178_RS17915 (MN178_17915)	3836712..3837263	+	552	WP_016253541.1	helix-turn-helix domain-containing protein	-
MN178_RS17920 (MN178_17920)	3837260..3837658	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
MN178_RS17925 (MN178_17925)	3837671..3837994	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
MN178_RS17930 (MN178_17930)	3838325..3838786	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
MN178_RS17935 (MN178_17935)	3838791..3840986	+	2196	WP_023087567.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
MN178_RS17940 (MN178_17940)	3841144..3841698	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
MN178_RS17945 (MN178_17945)	3841787..3842365	+	579	WP_003102740.1	HD domain-containing protein	Toxin
MN178_RS17950 (MN178_17950)	3842474..3843661	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
MN178_RS17955 (MN178_17955)	3843651..3845822	-	2172	WP_003088105.1	type I secretion system permease/ATPase	-
MN178_RS17960 (MN178_17960)	3845812..3847089	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T238217 WP_003102740.1 NZ_CP093356:3841787-3842365 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T238217 NZ_LR890277:325912-326280 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT238217 WP_003088112.1 NZ_CP093356:3841144-3841698 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT238217 NZ_LR890277:325694-325915 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATATGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07