Detailed information of TA system TA238209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2789365..2790407
• Replicon: chromosome
• Accession: NZ_CP093355
• Organism: Pseudomonas aeruginosa strain E104

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LRN73_RS13330
• Protein ID: WP_003153636.1
• Coordinates: 2789832..2790407 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: LRN73_RS13325
• Protein ID: WP_003050245.1
• Coordinates: 2789365..2789835 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRN73_RS13290 (LRN73_13290)	2784757..2786175	-	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-
LRN73_RS13295 (LRN73_13295)	2786165..2787076	-	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
LRN73_RS13300 (LRN73_13300)	2787073..2787765	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
LRN73_RS13305 (LRN73_13305)	2787762..2788160	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
LRN73_RS13310 (LRN73_13310)	2788172..2788531	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
LRN73_RS13315 (LRN73_13315)	2788548..2788781	-	234	WP_003090170.1	TIGR03758 family integrating conjugative element protein	-
LRN73_RS13320 (LRN73_13320)	2788778..2789161	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
LRN73_RS13325 (LRN73_13325)	2789365..2789835	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
LRN73_RS13330 (LRN73_13330)	2789832..2790407	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
LRN73_RS13335 (LRN73_13335)	2790425..2791339	+	915	WP_023114497.1	AAA family ATPase	-
LRN73_RS13340 (LRN73_13340)	2791336..2791806	+	471	WP_023114496.1	type III CBASS phage resistance system CD-NTase-associated protein Cap8	-
LRN73_RS13345 (LRN73_13345)	2791803..2792303	+	501	WP_003090159.1	type III CBASS phage resistance system CD-NTase-associated protein Cap7	-
LRN73_RS13350 (LRN73_13350)	2792303..2793205	+	903	WP_003090158.1	CBASS oligonucleotide cyclase	-
LRN73_RS13355 (LRN73_13355)	2793244..2793969	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2672344..2836523	164179

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T238209 WP_003153636.1 NZ_CP093355:2789832-2790407 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T238209 NZ_LR890270:c4989877-4989770 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT238209 WP_003050245.1 NZ_CP093355:2789365-2789835 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT238209 NZ_LR890270:4989930-4989991 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: I3TV68