Detailed information of TA system TA238202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 2013708..2015216
• Replicon: chromosome
• Accession: NZ_CP093354
• Organism: Pseudomonas aeruginosa strain E113

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9HYX1
• Locus tag: MN130_RS09460
• Protein ID: WP_003119682.1
• Coordinates: 2013708..2014295 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A9E8LAI1
• Locus tag: MN130_RS09465
• Protein ID: WP_003117606.1
• Coordinates: 2014362..2015216 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN130_RS09455 (MN130_09455)	2009963..2013442	-	3480	WP_003117605.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
MN130_RS09460 (MN130_09460)	2013708..2014295	+	588	WP_003119682.1	GNAT family protein	Toxin
MN130_RS09465 (MN130_09465)	2014362..2015216	+	855	WP_003117606.1	AraC family transcriptional regulator	Antitoxin
MN130_RS09470 (MN130_09470)	2015313..2017478	+	2166	WP_003091606.1	TonB-dependent receptor family protein	-
MN130_RS09475 (MN130_09475)	2017653..2019527	+	1875	WP_003117607.1	MFS transporter	-
MN130_RS09480 (MN130_09480)	2019588..2019797	-	210	WP_003115568.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21901.23 Da
• Isoelectric Point: 9.8676

>T238202 WP_003119682.1 NZ_CP093354:2013708-2014295 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T238202 NZ_LR890270:3700208-3700396 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGTAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCAGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCTCCAGAACGGCTTAACA
CAATCCTTTTACTGGTAGACGGCTCATGA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31863.14 Da
• Isoelectric Point: 7.5295

>AT238202 WP_003117606.1 NZ_CP093354:2014362-2015216 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTTYE

Nucleotide

• Length: 855 bp

>AT238202 NZ_LR890270:3699972-3700205 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• PDB: 1YRE
• PDB: 8GXJ