Detailed information of TA system TA238201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1743347..1745042
• Replicon: chromosome
• Accession: NZ_CP093354
• Organism: Pseudomonas aeruginosa strain E113

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: MN130_RS08205
• Protein ID: WP_003112901.1
• Coordinates: 1743347..1744216 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: MN130_RS08210
• Protein ID: WP_003112902.1
• Coordinates: 1744209..1745042 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN130_RS08180 (MN130_08180)	1739355..1740206	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
MN130_RS08185 (MN130_08185)	1740247..1741254	+	1008	WP_003162724.1	ABC transporter substrate-binding protein	-
MN130_RS08190 (MN130_08190)	1741251..1742027	+	777	WP_003112898.1	ABC transporter permease	-
MN130_RS08195 (MN130_08195)	1742033..1742794	+	762	WP_003112899.1	SDR family oxidoreductase	-
MN130_RS08200 (MN130_08200)	1742808..1743338	+	531	WP_003112900.1	cupin domain-containing protein	-
MN130_RS08205 (MN130_08205)	1743347..1744216	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
MN130_RS08210 (MN130_08210)	1744209..1745042	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
MN130_RS08215 (MN130_08215)	1745029..1745826	+	798	WP_017001845.1	SDR family oxidoreductase	-
MN130_RS08220 (MN130_08220)	1745819..1747501	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
MN130_RS08225 (MN130_08225)	1747512..1748315	+	804	WP_003453564.1	aspartate dehydrogenase	-
MN130_RS08230 (MN130_08230)	1748327..1749811	+	1485	WP_031756606.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T238201 WP_003112901.1 NZ_CP093354:1743347-1744216 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T238201 NZ_LR890270:3587507-3587794 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT238201 WP_003112902.1 NZ_CP093354:1744209-1745042 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT238201 NZ_LR890270:3587781-3588083 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1