Detailed information of TA system TA238197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 983567..984053
• Replicon: chromosome
• Accession: NZ_CP093337
• Organism: Shinella sp. H4-D48

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: MNR02_RS04690
• Protein ID: WP_241948263.1
• Coordinates: 983784..984053 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: MNR02_RS04685
• Protein ID: WP_241948262.1
• Coordinates: 983567..983800 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNR02_RS04670 (MNR02_04670)	979050..980138	+	1089	WP_134646944.1	hypothetical protein	-
MNR02_RS04675 (MNR02_04675)	980389..981537	+	1149	WP_241948260.1	hypothetical protein	-
MNR02_RS04680 (MNR02_04680)	981587..983497	+	1911	WP_241948261.1	AAA family ATPase	-
MNR02_RS04685 (MNR02_04685)	983567..983800	+	234	WP_241948262.1	DUF6290 family protein	Antitoxin
MNR02_RS04690 (MNR02_04690)	983784..984053	+	270	WP_241948263.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MNR02_RS04695 (MNR02_04695)	984072..985373	+	1302	WP_241948264.1	hypothetical protein	-
MNR02_RS04700 (MNR02_04700)	985366..986514	+	1149	WP_241948265.1	DUF2333 family protein	-
MNR02_RS04705 (MNR02_04705)	986735..988414	+	1680	WP_241948266.1	formate--tetrahydrofolate ligase	-
MNR02_RS04710 (MNR02_04710)	988453..988959	-	507	WP_241948267.1	DUF1993 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10182.84 Da
• Isoelectric Point: 10.1380

>T238197 WP_241948263.1 NZ_CP093337:983784-984053 [Shinella sp. H4-D48]
MAWAIEITEGAKKELDKLGHVEAKRILNFLVTKLASSENPRLLGSALQGARLGSYWRYRVGDYRIICDIQDNRLVVVVVR
VGHRREIYS

Nucleotide

• Length: 270 bp

>T238197 NZ_LR890270:2161285-2161749 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8994.96 Da
• Isoelectric Point: 4.3633

>AT238197 WP_241948262.1 NZ_CP093337:983567-983800 [Shinella sp. H4-D48]
MARQTAIRLPDETYERLQALAARTGRTATYYIREAIEQHIEDLEDIYLAEQELEHVRRGESRVYSLKEVEDELGLGD

Nucleotide

• Length: 234 bp

>AT238197 NZ_LR890270:2160950-2161285 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA