Detailed information of TA system TA238196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 5225441..5226076
• Replicon: chromosome
• Accession: NZ_CP093336
• Organism: Paenibacillus sp. N3/727

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: MNQ98_RS24305
• Protein ID: WP_208918011.1
• Coordinates: 5225441..5225791 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A268EX94
• Locus tag: MNQ98_RS24310
• Protein ID: WP_046681343.1
• Coordinates: 5225795..5226076 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNQ98_RS24285 (MNQ98_24285)	5221392..5221862	-	471	WP_241934027.1	SprT family protein	-
MNQ98_RS24290 (MNQ98_24290)	5222066..5222416	+	351	WP_241934028.1	hydrolase/acyltransferase	-
MNQ98_RS24295 (MNQ98_24295)	5222497..5222625	+	129	WP_208918012.1	cortex morphogenetic protein CmpA	-
MNQ98_RS24300 (MNQ98_24300)	5222807..5225017	-	2211	WP_277883692.1	Tex family protein	-
MNQ98_RS24305 (MNQ98_24305)	5225441..5225791	-	351	WP_208918011.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MNQ98_RS24310 (MNQ98_24310)	5225795..5226076	-	282	WP_046681343.1	ribbon-helix-helix protein, CopG family	Antitoxin
MNQ98_RS24315 (MNQ98_24315)	5226310..5227488	-	1179	WP_241930539.1	alanine racemase	-
MNQ98_RS24320 (MNQ98_24320)	5227765..5228871	-	1107	WP_241930540.1	outer membrane lipoprotein carrier protein LolA	-
MNQ98_RS24325 (MNQ98_24325)	5229058..5230383	-	1326	WP_241934030.1	MFS transporter	-
MNQ98_RS24330 (MNQ98_24330)	5230415..5230915	-	501	WP_241930541.1	winged helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-46)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12719.58 Da
• Isoelectric Point: 4.8384

>T238196 WP_208918011.1 NZ_CP093336:c5225791-5225441 [Paenibacillus sp. N3/727]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEETMSKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T238196 NZ_LR890270:c2118584-2118270 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAATCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10757.33 Da
• Isoelectric Point: 6.4708

>AT238196 WP_046681343.1 NZ_CP093336:c5226076-5225795 [Paenibacillus sp. N3/727]
VANMQNTKRIMISLPDHLLQEVDGIAQLENSNRSELIRQAMKLYLNERKKRYIRESMQRGYMEMAKINLTMASEAFHAEE
DADSTLDRLVSGV

Nucleotide

• Length: 282 bp

>AT238196 NZ_LR890270:c2118273-2117857 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACGCCCAGGCGCTGGAACTGGTCGATCATCTGCTGCTCAACGATCCTGAAAATCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATTGGCAGTAAATCCATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A268EX94