Detailed information of TA system TA023818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 821356..821962
• Replicon: chromosome
• Accession: NC_012726
• Organism: Sulfolobus islandicus M.16.4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C4KFX2
• Locus tag: M164_RS04310
• Protein ID: WP_012735761.1
• Coordinates: 821570..821962 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C4KFX1
• Locus tag: M164_RS04305
• Protein ID: WP_012710923.1
• Coordinates: 821356..821580 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M164_RS04295 (M164_0870)	817989..818753	+	765	WP_012710921.1	enoyl-CoA hydratase/isomerase family protein	-
M164_RS04300 (M164_0871)	818756..821023	+	2268	WP_012735760.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
M164_RS04305 (M164_0872)	821356..821580	+	225	WP_012710923.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
M164_RS04310 (M164_0873)	821570..821962	+	393	WP_012735761.1	type II toxin-antitoxin system VapC family toxin	Toxin
M164_RS04315 (M164_0874)	822010..822354	-	345	Protein_881	ISNCY family transposase	-
M164_RS04320 (M164_0875)	822576..823385	-	810	WP_012735763.1	type III-B CRISPR module-associated Cmr3 family protein	-
M164_RS04325 (M164_0876)	823391..826060	-	2670	WP_012735764.1	type III-B CRISPR-associated protein Cas10/Cmr2	-
M164_RS04330 (M164_0877)	826045..826773	-	729	WP_012735765.1	type III-B CRISPR module RAMP protein Cmr6	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (9-124)

Antitoxin

• Post-segregation antitoxin CcdA (5-54)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 15077.81 Da
• Isoelectric Point: 8.2956

>T23818 WP_012735761.1 NC_012726:821570-821962 [Sulfolobus islandicus M.16.4]
MKDKEFLFDASALYSLLDYVDKLDVKKIHILTLTFYEVGNVIWKEYYIHKKVKDPITLSMLFHKLMRKLNVVEDPPLDGV
MRIAIERGLTYYDASYAYVAESLGLILVSNDKELIRKANAISLKDLIKSM

Nucleotide

• Length: 393 bp

>T23818 NC_012726:821570-821962 [Sulfolobus islandicus M.16.4]
ATGAAAGATAAGGAATTCCTATTCGATGCCTCAGCTTTGTACTCGCTTTTAGATTACGTGGATAAGTTAGATGTTAAGAA
AATTCATATACTTACCCTAACTTTTTATGAGGTAGGCAACGTCATATGGAAGGAGTATTATATACACAAAAAGGTTAAAG
ATCCTATAACTCTTTCGATGCTTTTCCATAAATTAATGCGAAAACTTAACGTAGTAGAGGATCCGCCTCTTGATGGGGTA
ATGAGAATTGCCATAGAAAGGGGTTTAACTTACTATGACGCATCTTATGCGTATGTTGCTGAATCTTTAGGGCTTATCCT
AGTGTCTAACGATAAAGAGCTGATAAGAAAAGCCAATGCCATTTCGCTAAAGGATTTGATAAAAAGTATGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8796.18 Da
• Isoelectric Point: 5.3410

>AT23818 WP_012710923.1 NC_012726:821356-821580 [Sulfolobus islandicus M.16.4]
MSDVISVRVKKELKKKAEELGINIREVVEKALEEAIKEKEKEELKDIAMRIKELMRDVSENDWVRAVRESRDER

Nucleotide

• Length: 225 bp

>AT23818 NC_012726:821356-821580 [Sulfolobus islandicus M.16.4]
ATGTCAGACGTAATAAGCGTAAGAGTGAAGAAAGAGCTGAAGAAGAAGGCAGAGGAGTTGGGAATTAACATTAGGGAAGT
TGTAGAAAAAGCTTTAGAGGAAGCCATAAAGGAGAAGGAGAAGGAAGAGCTTAAGGATATAGCTATGAGAATTAAGGAAC
TAATGAGGGATGTAAGTGAAAATGATTGGGTAAGGGCTGTTAGGGAGAGTAGAGATGAAAGATAA

Structures

Toxin

• AlphaFold DB: C4KFX2

Antitoxin

• AlphaFold DB: C4KFX1