Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1840122..1840741 Replicon chromosome
Accession NZ_CP093334
Organism Raoultella planticola strain A2-F21

Toxin (Protein)


Gene name Hha Uniprot ID A0A514EV16
Locus tag MNO12_RS09040 Protein ID WP_004858783.1
Coordinates 1840523..1840741 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A514EV88
Locus tag MNO12_RS09035 Protein ID WP_004858785.1
Coordinates 1840122..1840496 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNO12_RS09025 (MNO12_09025) 1835232..1836425 + 1194 WP_032689203.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MNO12_RS09030 (MNO12_09030) 1836448..1839594 + 3147 WP_032689204.1 multidrug efflux RND transporter permease subunit AcrB -
MNO12_RS09035 (MNO12_09035) 1840122..1840496 + 375 WP_004858785.1 Hha toxicity modulator TomB Antitoxin
MNO12_RS09040 (MNO12_09040) 1840523..1840741 + 219 WP_004858783.1 HHA domain-containing protein Toxin
MNO12_RS09045 (MNO12_09045) 1840893..1841459 + 567 WP_032689205.1 maltose O-acetyltransferase -
MNO12_RS09050 (MNO12_09050) 1841431..1841565 - 135 WP_223306663.1 hypothetical protein -
MNO12_RS09055 (MNO12_09055) 1841559..1842029 + 471 WP_004858780.1 YlaC family protein -
MNO12_RS09060 (MNO12_09060) 1842004..1843455 - 1452 WP_241950842.1 PLP-dependent aminotransferase family protein -
MNO12_RS09065 (MNO12_09065) 1843557..1844267 + 711 WP_241950843.1 GNAT family protein -
MNO12_RS09070 (MNO12_09070) 1844264..1844404 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
MNO12_RS09075 (MNO12_09075) 1844407..1844667 - 261 WP_032689208.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8570.90 Da        Isoelectric Point: 7.9907

>T238179 WP_004858783.1 NZ_CP093334:1840523-1840741 [Raoultella planticola]
MSGEPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T238179 NZ_LR890254:c3289172-3288804 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14411.19 Da        Isoelectric Point: 4.8989

>AT238179 WP_004858785.1 NZ_CP093334:1840122-1840496 [Raoultella planticola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYAEDNKLIAQLDEYL
DDTFMLFSSYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT238179 NZ_LR890254:c3289390-3289169 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EV16

Antitoxin

Source ID Structure
AlphaFold DB A0A514EV88

References