Detailed information of TA system TA238178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 269171..270774
• Replicon: chromosome
• Accession: NZ_CP093334
• Organism: Raoultella planticola strain A2-F21

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A8G2A023
• Locus tag: MNO12_RS01550
• Protein ID: WP_032687736.1
• Coordinates: 269171..270502 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2X2EM10
• Locus tag: MNO12_RS01555
• Protein ID: WP_032696205.1
• Coordinates: 270502..270774 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNO12_RS01535 (MNO12_01535)	265047..265961	-	915	WP_015584333.1	branched-chain amino acid ABC transporter permease	-
MNO12_RS01540 (MNO12_01540)	266061..267119	-	1059	WP_162180097.1	branched-chain amino acid ABC transporter substrate-binding protein	-
MNO12_RS01545 (MNO12_01545)	267832..269238	+	1407	WP_032699891.1	glycoside hydrolase family 10 protein	-
MNO12_RS01550 (MNO12_01550)	269171..270502	-	1332	WP_032687736.1	type II toxin-antitoxin system HipA family toxin	Toxin
MNO12_RS01555 (MNO12_01555)	270502..270774	-	273	WP_032696205.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
MNO12_RS01560 (MNO12_01560)	270968..271522	-	555	WP_046852510.1	RNA polymerase sigma factor	-
MNO12_RS01565 (MNO12_01565)	271528..271953	-	426	WP_032687741.1	hypothetical protein	-
MNO12_RS01570 (MNO12_01570)	272085..273038	-	954	WP_032687742.1	hypothetical protein	-
MNO12_RS01575 (MNO12_01575)	273254..273886	-	633	WP_032699859.1	class I SAM-dependent methyltransferase	-
MNO12_RS01580 (MNO12_01580)	273906..275147	-	1242	WP_032699861.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 49482.82 Da
• Isoelectric Point: 6.6648

>T238178 WP_032687736.1 NZ_CP093334:c270502-269171 [Raoultella planticola]
MATLVTWMNNERVGELIKQANGAHLFRYEEAWMRNPRARPLSLSLPLQYGNITDDAVFNYFDNLLPDSPRVRDRIVKRYQ
ARSKQPFDLLAEIGRDSVGAVTLLPPGENATRRALSWEALDSHALDALLTAYQSDIPLGMVNEHDDFRISVAGAQEKTAL
LKIGEQWCIPTGTTPTTHIIKLPIGEIKQPDAVLDLSESVDNEYLCLALARALGFAVPRASIIQTASIRALAVERFDRRW
AQEKTVLLRLPQEDLCQAFGIPSSIKYESDGGPGIRAIMAFLVGSSEALEDRYNFMKFMVFQWLIGATDGHAKNFSIFLQ
PGGSYRLTPFYDIISAFPLLGGKGLHLSDLRLSMSLKASKGRKTEIQTLYPRHFLATAKEVGFAEEQMLEILRYFVDNVP
HAVAAVKETLPHDFSPPVYEAITDRLLWVHSRLQNAMAALPSG

Nucleotide

• Length: 1332 bp

>T238178 NZ_LR890254:c2801742-2801332 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATAGCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10168.43 Da
• Isoelectric Point: 4.9491

>AT238178 WP_032696205.1 NZ_CP093334:c270774-270502 [Raoultella planticola]
MSPSPGIYSPVQLANYLKLVRQKNQWTQSDLAQRMGIKQATVSNFENHPEKTTLTTVFKILQSLELSMAICEKNAPDTND
SSDNPQDLDW

Nucleotide

• Length: 273 bp

>AT238178 NZ_LR890254:c2801989-2801723 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X2EM10