Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1864183..1864787 Replicon chromosome
Accession NZ_CP093333
Organism Pseudomonas simiae strain H4-B59

Toxin (Protein)


Gene name hicA Uniprot ID A0A1N7UCS2
Locus tag MNO08_RS08515 Protein ID WP_010211777.1
Coordinates 1864183..1864371 (+) Length 63 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1N7UII5
Locus tag MNO08_RS08520 Protein ID WP_010211776.1
Coordinates 1864383..1864787 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNO08_RS08480 (MNO08_08480) 1859913..1860449 + 537 WP_010211784.1 DsbE family thiol:disulfide interchange protein -
MNO08_RS08485 (MNO08_08485) 1860446..1860919 + 474 WP_012723038.1 cytochrome c-type biogenesis protein CcmH -
MNO08_RS08490 (MNO08_08490) 1860912..1862099 + 1188 WP_187517437.1 c-type cytochrome biogenesis protein CcmI -
MNO08_RS08495 (MNO08_08495) 1862116..1862511 + 396 WP_010211781.1 hypothetical protein -
MNO08_RS08500 (MNO08_08500) 1862600..1862737 + 138 Protein_1675 alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase -
MNO08_RS08505 (MNO08_08505) 1862790..1863362 + 573 WP_010211780.1 phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN -
MNO08_RS08510 (MNO08_08510) 1863387..1863863 - 477 WP_010211779.1 GNAT family N-acetyltransferase -
MNO08_RS08515 (MNO08_08515) 1864183..1864371 + 189 WP_010211777.1 type II toxin-antitoxin system HicA family toxin Toxin
MNO08_RS08520 (MNO08_08520) 1864383..1864787 + 405 WP_010211776.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MNO08_RS08525 (MNO08_08525) 1865093..1866649 + 1557 WP_106120161.1 M10 family metallopeptidase C-terminal domain-containing protein -
MNO08_RS08530 (MNO08_08530) 1866659..1867855 - 1197 WP_021492219.1 MFS transporter -
MNO08_RS08535 (MNO08_08535) 1868032..1868646 + 615 WP_021492218.1 DUF5666 domain-containing protein -
MNO08_RS08540 (MNO08_08540) 1868776..1869780 - 1005 WP_010211770.1 sulfate ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7161.38 Da        Isoelectric Point: 11.0014

>T238176 WP_010211777.1 NZ_CP093333:1864183-1864371 [Pseudomonas simiae]
VQSRLLIKELQEAGWVLDRVAGSHHIFTHRYNPYTIPVPHPKKDLPLGTVKSIRKRAGLFQF

Download         Length: 189 bp

>T238176 NZ_LR890254:810355-811677 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCACCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14683.64 Da        Isoelectric Point: 4.6000

>AT238176 WP_010211776.1 NZ_CP093333:1864383-1864787 [Pseudomonas simiae]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGPIPLPTSVANHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Download         Length: 405 bp

>AT238176 NZ_LR890254:810089-810355 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCACTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1N7UCS2

Antitoxin

Source ID Structure
AlphaFold DB A0A1N7UII5

References