Detailed information of TA system TA238162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2336418..2337196
• Replicon: chromosome
• Accession: NZ_CP093332
• Organism: Buttiauxella ferragutiae strain H4-C11

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: MNO13_RS10755
• Protein ID: WP_064545897.1
• Coordinates: 2336708..2337196 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A4R6EAM7
• Locus tag: MNO13_RS10750
• Protein ID: WP_064545895.1
• Coordinates: 2336418..2336711 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNO13_RS10720 (MNO13_10720)	2332127..2332570	+	444	WP_064545887.1	dihydroneopterin triphosphate diphosphatase	-
MNO13_RS10725 (MNO13_10725)	2332594..2333337	+	744	WP_241939970.1	YebC/PmpR family DNA-binding transcriptional regulator	-
MNO13_RS10730 (MNO13_10730)	2333381..2333902	+	522	WP_064545890.1	crossover junction endodeoxyribonuclease RuvC	-
MNO13_RS10735 (MNO13_10735)	2333982..2334593	+	612	WP_064545891.1	Holliday junction branch migration protein RuvA	-
MNO13_RS10740 (MNO13_10740)	2334615..2335625	+	1011	WP_064545893.1	Holliday junction branch migration DNA helicase RuvB	-
MNO13_RS10745 (MNO13_10745)	2336178..2336327	+	150	WP_133521653.1	type I addiction module toxin, SymE family	-
MNO13_RS10750 (MNO13_10750)	2336418..2336711	+	294	WP_064545895.1	DUF1778 domain-containing protein	Antitoxin
MNO13_RS10755 (MNO13_10755)	2336708..2337196	+	489	WP_064545897.1	GNAT family N-acetyltransferase	Toxin
MNO13_RS10760 (MNO13_10760)	2337272..2338057	-	786	WP_064545898.1	zinc ABC transporter permease subunit ZnuB	-
MNO13_RS10765 (MNO13_10765)	2338054..2338809	-	756	WP_064545899.1	zinc ABC transporter ATP-binding protein ZnuC	-
MNO13_RS10770 (MNO13_10770)	2338889..2339833	+	945	WP_064545900.1	zinc ABC transporter substrate-binding protein ZnuA	-
MNO13_RS10775 (MNO13_10775)	2339849..2341171	+	1323	WP_074388553.1	murein DD-endopeptidase MepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-94)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17543.44 Da
• Isoelectric Point: 7.6433

>T238162 WP_064545897.1 NZ_CP093332:2336708-2337196 [Buttiauxella ferragutiae]
MIAAPEPLRAEHVVSQFCCGVESMDSWLRQRAMKNQLTGASRTFVCCDEARVLAYYSLASSAVAPNSAPGRFRRNMPDPI
PVVILGCLAVDKSLHGKGIGRALVRDAGLRVIQTAETIGIRGMLVHALSEEARDFYLRVGFEPSPVEPMTLMITTGDLVA
AL

Nucleotide

• Length: 489 bp

>T238162 NZ_LR890240:c4676244-4675960 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11015.63 Da
• Isoelectric Point: 10.1968

>AT238162 WP_064545895.1 NZ_CP093332:2336418-2336711 [Buttiauxella ferragutiae]
MPGAQNIGVKRETLNLRIKSTERDLIDRAAKVRGKNRTDFVLDAARAAAEETLLEQRIIMVDPEAYQEFLARLDQAPESN
AALRKTMQTIAPWNHKK

Nucleotide

• Length: 294 bp

>AT238162 NZ_LR890240:c4676476-4676234 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A4R6EAM7