Detailed information of TA system TA238156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/ElaA-DUF1778
• Location: 61246..61982
• Replicon: plasmid p1
• Accession: NZ_CP093329
• Organism: Rahnella aceris strain S2-A69

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: H8P154
• Locus tag: MNO10_RS22075
• Protein ID: WP_014683045.1
• Coordinates: 61246..61725 (-)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: MNO10_RS22080
• Protein ID: WP_200227549.1
• Coordinates: 61722..61982 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNO10_RS22055 (MNO10_22055)	57380..58966	-	1587	WP_013578364.1	sensor domain-containing diguanylate cyclase	-
MNO10_RS22060 (MNO10_22060)	59287..59553	-	267	WP_013578404.1	Txe/YoeB family addiction module toxin	-
MNO10_RS22065 (MNO10_22065)	59550..59795	-	246	WP_013578405.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MNO10_RS22070 (MNO10_22070)	60105..61154	+	1050	WP_014683046.1	tyrosine-type recombinase/integrase	-
MNO10_RS22075 (MNO10_22075)	61246..61725	-	480	WP_014683045.1	GNAT family N-acetyltransferase	Toxin
MNO10_RS22080 (MNO10_22080)	61722..61982	-	261	WP_200227549.1	DUF1778 domain-containing protein	Antitoxin
MNO10_RS22085 (MNO10_22085)	62134..62475	+	342	WP_241917281.1	MFS transporter	-
MNO10_RS22090 (MNO10_22090)	62823..64283	+	1461	WP_014683042.1	sensor domain-containing diguanylate cyclase	-
MNO10_RS22095 (MNO10_22095)	64756..65535	-	780	WP_200227563.1	SDR family oxidoreductase	-
MNO10_RS22100 (MNO10_22100)	65535..65861	-	327	WP_241917282.1	nuclear transport factor 2 family protein	-
MNO10_RS22105 (MNO10_22105)	65952..66278	+	327	WP_112152208.1	helix-turn-helix domain-containing protein	-
MNO10_RS22110 (MNO10_22110)	66422..66762	-	341	Protein_69	iron-containing alcohol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..123458	123458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (26-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-85)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17323.02 Da
• Isoelectric Point: 9.5460

>T238156 WP_014683045.1 NZ_CP093329:c61725-61246 [Rahnella aceris]
MSLQLSAPEPLTTAHILDAFGCGETTLDEWLKRRALTNHLNGASRTFVVTAHDKSVVGYYALAAGAVSHREAAGSVRRNM
PDPIPVIVLGRLAVDLRTQGVKLGSSLLQDAVLRVRSIVENTGVRALLVHALHEPAKQFYEHYGFTSSPINPMTLMLRL

Nucleotide

• Length: 480 bp

>T238156 NZ_LR890240:781803-782213 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9741.18 Da
• Isoelectric Point: 4.7483

>AT238156 WP_200227549.1 NZ_CP093329:c61982-61722 [Rahnella aceris]
MRDAAINLRALPEQRDLIDQAASLLGKNRSDFMLEVACERAQAILLDQVFFQLDTEKFHEFTAMLDAPHNANQGLERLMA
VKAPWA

Nucleotide

• Length: 261 bp

>AT238156 NZ_LR890240:781556-781822 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: H8P154