Detailed information of TA system TA023815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 123784..124421
• Replicon: plasmid bglu_4p
• Accession: NC_012725
• Organism: Burkholderia glumae BGR1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C5AP38
• Locus tag: BGLU_RS31235
• Protein ID: WP_012735347.1
• Coordinates: 123784..124185 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: C5AP39
• Locus tag: BGLU_RS31240
• Protein ID: WP_012735348.1
• Coordinates: 124185..124421 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BGLU_RS33720	120247..120516	+	270	WP_012735343.1	hypothetical protein	-
BGLU_RS31215	120676..121290	+	615	Protein_108	error-prone DNA polymerase	-
BGLU_RS33725	121306..121593	+	288	WP_012735344.1	hypothetical protein	-
BGLU_RS35255	122697..122891	-	195	WP_124837731.1	hypothetical protein	-
BGLU_RS31225	122946..123188	-	243	WP_012735345.1	hypothetical protein	-
BGLU_RS31230	123206..123769	-	564	WP_012735346.1	hypothetical protein	-
BGLU_RS31235	123784..124185	-	402	WP_012735347.1	type II toxin-antitoxin system VapC family toxin	Toxin
BGLU_RS31240	124185..124421	-	237	WP_012735348.1	AbrB family transcriptional regulator	Antitoxin
BGLU_RS31245	124758..125450	-	693	WP_043308722.1	hypothetical protein	-
BGLU_RS31250	125534..127360	-	1827	WP_012735349.1	site-specific integrase	-
BGLU_RS31255	127357..127737	-	381	WP_080569442.1	hypothetical protein	-
BGLU_RS31260	127740..128057	-	318	WP_050811486.1	hypothetical protein	-
BGLU_RS31265	128108..129196	-	1089	Protein_119	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..134346	134346

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14847.12 Da
• Isoelectric Point: 5.2138

>T23815 WP_012735347.1 NC_012725:c124185-123784 [Burkholderia glumae BGR1]
MPRFMLDTNMCIYLMKNQPEQVAKRFAECFVGDVVMSAITYAELEYGVAVSDNRAKERRNLAALIEDILVAPFDAAAAVA
YGPIREATRERKKDHLDKLIAAHAVALDVVLVTNNAKDFASYPGIKLDNWLND

Nucleotide

• Length: 402 bp

>T23815 NC_012725:c124185-123784 [Burkholderia glumae BGR1]
ATGCCGCGCTTCATGCTCGACACCAATATGTGCATCTACCTGATGAAGAACCAGCCTGAGCAGGTAGCAAAGCGTTTCGC
GGAGTGCTTCGTCGGTGACGTGGTGATGTCCGCTATCACATATGCCGAGCTTGAATACGGTGTCGCGGTTTCTGACAACC
GGGCGAAAGAGCGGCGGAACCTCGCTGCGTTGATTGAGGACATTCTGGTTGCTCCGTTTGATGCTGCTGCGGCTGTAGCC
TATGGTCCGATTCGCGAGGCTACACGGGAGCGCAAGAAGGACCACCTAGACAAGTTGATCGCCGCCCATGCAGTTGCGCT
TGACGTGGTGCTTGTCACAAACAACGCGAAGGACTTCGCAAGCTATCCGGGTATCAAGCTCGACAACTGGCTGAATGACT
GA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8983.13 Da
• Isoelectric Point: 6.3676

>AT23815 WP_012735348.1 NC_012725:c124421-124185 [Burkholderia glumae BGR1]
MQTTRVFRNGNSQAVRIPADLAYERSDVELEIERVGDEIRIRPARRPLTGVLKKFAKFSQDFMAEGRGEQEQAEREGM

Nucleotide

• Length: 237 bp

>AT23815 NC_012725:c124421-124185 [Burkholderia glumae BGR1]
ATGCAGACAACCCGAGTTTTCAGGAACGGCAACTCTCAGGCCGTTCGCATCCCCGCCGATCTTGCCTATGAGCGCAGCGA
CGTTGAGCTTGAAATCGAGCGCGTGGGCGACGAAATCCGCATCCGACCGGCTCGACGTCCCTTGACGGGGGTACTCAAGA
AATTTGCCAAATTCAGCCAAGATTTCATGGCCGAGGGGCGCGGCGAGCAGGAACAAGCTGAACGTGAGGGTATGTAA

Structures

Toxin

• AlphaFold DB: C5AP38

Antitoxin

• AlphaFold DB: C5AP39