Detailed information of TA system TA238141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 149840..150457
• Replicon: chromosome
• Accession: NZ_CP093328
• Organism: Rahnella aceris strain S2-A69

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H8NYI7
• Locus tag: MNO10_RS00730
• Protein ID: WP_013576617.1
• Coordinates: 149840..150043 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: H8NYI6
• Locus tag: MNO10_RS00735
• Protein ID: WP_013576616.1
• Coordinates: 150089..150457 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNO10_RS00715 (MNO10_00715)	145455..146804	-	1350	WP_119261884.1	NCS2 family permease	-
MNO10_RS00720 (MNO10_00720)	146881..148668	-	1788	WP_241917002.1	adenine deaminase C-terminal domain-containing protein	-
MNO10_RS00725 (MNO10_00725)	148808..149764	+	957	WP_013576618.1	LysR family transcriptional regulator	-
MNO10_RS00730 (MNO10_00730)	149840..150043	-	204	WP_013576617.1	HHA domain-containing protein	Toxin
MNO10_RS00735 (MNO10_00735)	150089..150457	-	369	WP_013576616.1	Hha toxicity modulator TomB	Antitoxin
MNO10_RS00740 (MNO10_00740)	150687..151040	-	354	WP_013576615.1	hypothetical protein	-
MNO10_RS00745 (MNO10_00745)	151390..152151	+	762	WP_241917003.1	ABC transporter ATP-binding protein	-
MNO10_RS00750 (MNO10_00750)	152148..153005	+	858	WP_013576613.1	metal ABC transporter permease	-
MNO10_RS00755 (MNO10_00755)	153049..153927	+	879	WP_013576612.1	metal ABC transporter substrate-binding protein	-
MNO10_RS00760 (MNO10_00760)	154052..154192	-	141	WP_013576611.1	type B 50S ribosomal protein L36	-
MNO10_RS00765 (MNO10_00765)	154213..154467	-	255	WP_013576610.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8114.45 Da
• Isoelectric Point: 6.9764

>T238141 WP_013576617.1 NZ_CP093328:c150043-149840 [Rahnella aceris]
MNKIDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPVSVWKFVR

Nucleotide

• Length: 204 bp

>T238141 NZ_LR890235:324877-325245 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGATGCTAAACAAAATTGAATATGAAGGTGTGACAGATGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14391.15 Da
• Isoelectric Point: 4.7281

>AT238141 WP_013576616.1 NZ_CP093328:c150457-150089 [Rahnella aceris]
MDEYSPKRHDIAQLRYLNEMLYDEGIATLGDSHHGWVNDPTSAVNLQLNELIEHIASFVMSFKIKYPDDNHLSDLVEEYL
DDTYTLFSNYGINDSDLRQWQKTKKRLFRMFSGDYICTLMKT

Nucleotide

• Length: 369 bp

>AT238141 NZ_LR890235:324659-324880 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: H8NYI7

Antitoxin

• AlphaFold DB: H8NYI6