Detailed information of TA system TA238138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 3103635..3104592
• Replicon: plasmid p1
• Accession: NZ_CP093325
• Organism: Bacillus sp. N5-665 strain N5/665

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: MNO09_RS18430
• Protein ID: WP_116480647.1
• Coordinates: 3103855..3104592 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: MNO09_RS18425
• Protein ID: WP_000588712.1
• Coordinates: 3103635..3103760 (-)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNO09_RS18405 (MNO09_18405)	3099594..3100253	-	660	WP_000682543.1	CatB-related O-acetyltransferase	-
MNO09_RS18410 (MNO09_18410)	3100708..3101178	+	471	WP_000771847.1	DUF5065 family protein	-
MNO09_RS18415 (MNO09_18415)	3101772..3103241	-	1470	WP_000287560.1	beta-Ala-His dipeptidase	-
MNO09_RS18420 (MNO09_18420)	3103383..3103559	-	177	WP_000852619.1	stage II sporulation protein SB	-
MNO09_RS18425 (MNO09_18425)	3103635..3103760	-	126	WP_000588712.1	hypothetical protein	Antitoxin
MNO09_RS18430 (MNO09_18430)	3103855..3104592	-	738	WP_116480647.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MNO09_RS18435 (MNO09_18435)	3104929..3105666	-	738	WP_070168825.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
MNO09_RS18440 (MNO09_18440)	3105834..3106316	-	483	WP_000191899.1	MarR family transcriptional regulator	-
MNO09_RS18445 (MNO09_18445)	3106433..3108109	+	1677	WP_070168824.1	alpha-keto acid decarboxylase family protein	-
MNO09_RS18450 (MNO09_18450)	3108159..3108566	-	408	WP_070168823.1	VOC family protein	-
MNO09_RS18455 (MNO09_18455)	3108687..3109397	-	711	WP_070168822.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaZ / fosB1	bpsC / cap8E / ugd / bpsX / clpP / BAS3109 / bpsC / lap / inhA / clpP / hblA / hblD / hblC / hblA / hblA / hblD / hblC / dhbB / dhbE / dhbC / dhbA / asbF / asbE / asbD / asbC / asbB / asbA / nheC / nheB / nheA / ilsA / inhA / icl / BC0671 / inhA / groEL / tufA / clpC	1..5519593	5519593

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28349.07 Da
• Isoelectric Point: 8.3034

>T238138 WP_116480647.1 NZ_CP093325:c3104592-3103855 [Bacillus sp. N5-665]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKMVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T238138 NZ_LR890230:c4831202-4830285 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGTTGAAGTGCTGAGTCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4970.40 Da
• Isoelectric Point: 3.9150

>AT238138 WP_000588712.1 NZ_CP093325:c3103760-3103635 [Bacillus sp. N5-665]
MIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT238138 NZ_LR890230:c4831966-4831208 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A366GQT1