Detailed information of TA system TA238117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3692860..3693479
• Replicon: chromosome
• Accession: NZ_CP093322
• Organism: Yersinia intermedia strain N6/293

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: Q66DR5
• Locus tag: MNQ97_RS17045
• Protein ID: WP_002208622.1
• Coordinates: 3693276..3693479 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A0T9M685
• Locus tag: MNQ97_RS17040
• Protein ID: WP_005182270.1
• Coordinates: 3692860..3693228 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNQ97_RS17010 (MNQ97_17010)	3688492..3688764	+	273	WP_032905607.1	type B 50S ribosomal protein L31	-
MNQ97_RS17015 (MNQ97_17015)	3688767..3688910	+	144	WP_002208618.1	type B 50S ribosomal protein L36	-
MNQ97_RS17020 (MNQ97_17020)	3689077..3689955	-	879	WP_005182278.1	metal ABC transporter substrate-binding protein	-
MNQ97_RS17025 (MNQ97_17025)	3689984..3690841	-	858	WP_050312412.1	metal ABC transporter permease	-
MNQ97_RS17030 (MNQ97_17030)	3690838..3691575	-	738	WP_050086076.1	ABC transporter ATP-binding protein	-
MNQ97_RS17035 (MNQ97_17035)	3692348..3692701	+	354	WP_050086001.1	hypothetical protein	-
MNQ97_RS17040 (MNQ97_17040)	3692860..3693228	+	369	WP_005182270.1	Hha toxicity modulator TomB	Antitoxin
MNQ97_RS17045 (MNQ97_17045)	3693276..3693479	+	204	WP_002208622.1	expression modulating protein YmoA	Toxin
MNQ97_RS17055 (MNQ97_17055)	3694409..3694714	+	306	WP_186004081.1	MGMT family protein	-
MNQ97_RS17060 (MNQ97_17060)	3694805..3695359	-	555	WP_050086003.1	YbaY family lipoprotein	-
MNQ97_RS17065 (MNQ97_17065)	3695618..3696478	+	861	WP_226715545.1	acyl-CoA thioesterase II	-
MNQ97_RS17070 (MNQ97_17070)	3696569..3697858	-	1290	WP_241916459.1	ammonium transporter AmtB	-
MNQ97_RS17075 (MNQ97_17075)	3697898..3698236	-	339	WP_002208627.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8063.31 Da
• Isoelectric Point: 6.4573

>T238117 WP_002208622.1 NZ_CP093322:3693276-3693479 [Yersinia intermedia]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Nucleotide

• Length: 204 bp

>T238117 NZ_LR890217:795020-795430 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14222.93 Da
• Isoelectric Point: 4.4314

>AT238117 WP_005182270.1 NZ_CP093322:3692860-3693228 [Yersinia intermedia]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYPDDGDLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKERLFRLFSGEYTCTLMKT

Nucleotide

• Length: 369 bp

>AT238117 NZ_LR890217:794773-795039 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 2K5S

Antitoxin

• AlphaFold DB: A0A0T9M685