Detailed information of TA system TA023811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3413318..3414871
• Replicon: chromosome
• Accession: NC_012691
• Organism: Tolumonas auensis DSM 9187

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C4LDU2
• Locus tag: TOLA_RS15985
• Protein ID: WP_015880152.1
• Coordinates: 3413573..3414871 (+)
• Length: 433 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: C4LDU1
• Locus tag: TOLA_RS15980
• Protein ID: WP_015880151.1
• Coordinates: 3413318..3413569 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOLA_RS15975	3410051..3413191	+	3141	WP_015880150.1	DEAD/DEAH box helicase	-
TOLA_RS15980	3413318..3413569	+	252	WP_015880151.1	helix-turn-helix domain-containing protein	Antitoxin
TOLA_RS15985	3413573..3414871	+	1299	WP_015880152.1	type II toxin-antitoxin system HipA family toxin	Toxin
TOLA_RS15990	3414916..3416208	-	1293	WP_015880153.1	cobyrinate a,c-diamide synthase	-
TOLA_RS15995	3416198..3417667	-	1470	WP_041609973.1	cobyric acid synthase	-
TOLA_RS16000	3417906..3418190	+	285	WP_015880155.1	putative addiction module antidote protein	-
TOLA_RS16005	3418193..3419122	-	930	WP_015880156.1	tyrosine recombinase XerC	-
TOLA_RS16010	3419115..3419813	-	699	WP_049759198.1	DUF484 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 433 a.a.
• Molecular weight: 47998.77 Da
• Isoelectric Point: 7.0216

>T23811 WP_015880152.1 NC_012691:3413573-3414871 [Tolumonas auensis DSM 9187]
MDKLTVAFNGIEVGTLTRHGSGAMEFHYNPDWLTRQGARAISLSLPLSEKIYRGVQVYNFFDNLLPDNEAIRARIQARFR
LASKQPFDLLASIGRDCIGAIQLYPENSVILPINQITAEPLEEAEIASLLMGYKLAPQGMAVETDDFRISLAGAQEKTAL
LWYREQWHRPQGSTPTSHIIKLPIGHLAQNNIDLSESSENEWLCLQIAKAFGFPVANAELARFGEQNTLVVERFDRRWSK
DGSWLMRLPQEDFCQALGIAPALKYESDGGPGIADGMKLLLGSQQANQDRETFFRSQILFWLLAAIDGHAKNFSLFIETG
SAYRMTPLYDIISAYPLMAAGSIPAKRARMAMAVTGKNRHFHWATIQPRHFESTAQKAGYSQDKAAELMADFKAGADAVL
TKVSGSLPANFPAHISDPIFDGIRRQTNKLPG

Nucleotide

• Length: 1299 bp

>T23811 NC_012691:3413573-3414871 [Tolumonas auensis DSM 9187]
ATGGATAAACTAACGGTTGCTTTCAATGGTATTGAGGTTGGCACGCTTACCCGCCACGGCTCCGGAGCTATGGAGTTTCA
CTACAATCCGGACTGGCTGACTCGTCAGGGAGCCCGTGCTATTTCGTTGTCGCTTCCGCTGTCAGAAAAAATTTATCGGG
GAGTGCAAGTCTATAATTTTTTCGATAACTTATTACCAGATAACGAAGCAATCCGAGCCCGGATACAAGCCCGATTCAGG
TTAGCATCAAAGCAACCTTTTGATTTACTTGCCAGTATCGGACGTGACTGTATCGGCGCTATTCAGCTATATCCCGAGAA
CTCAGTGATTCTGCCAATAAATCAAATAACAGCCGAACCACTGGAAGAGGCTGAAATAGCCAGTTTACTTATGGGGTACA
AACTCGCGCCGCAGGGAATGGCAGTAGAAACAGATGATTTCCGCATTTCGCTGGCCGGAGCCCAGGAGAAAACAGCCCTG
CTTTGGTATCGTGAACAATGGCACAGACCACAGGGCAGTACTCCGACCAGCCATATCATCAAGTTACCAATTGGCCATTT
AGCACAAAACAATATCGATCTTAGCGAAAGTAGTGAGAATGAATGGCTATGTCTGCAAATAGCTAAAGCATTCGGGTTTC
CCGTTGCGAATGCGGAACTGGCACGCTTTGGTGAACAGAACACCTTAGTTGTTGAACGGTTTGACCGCCGCTGGTCTAAA
GATGGTAGTTGGCTGATGCGTCTGCCACAGGAAGATTTTTGTCAGGCGTTAGGTATCGCACCGGCACTGAAATATGAATC
AGATGGCGGACCCGGCATAGCCGATGGAATGAAATTACTACTTGGTTCCCAACAAGCCAATCAAGATAGAGAAACCTTCT
TCAGGAGTCAGATTCTGTTCTGGTTGCTGGCTGCTATCGATGGTCATGCTAAAAATTTCAGCCTGTTTATTGAAACCGGC
AGCGCCTATCGTATGACTCCGCTCTACGACATTATTTCAGCTTACCCATTAATGGCTGCCGGAAGTATTCCCGCCAAACG
AGCTCGGATGGCAATGGCGGTTACAGGCAAAAACCGGCATTTCCACTGGGCAACAATCCAGCCGAGGCACTTTGAAAGTA
CCGCTCAGAAAGCTGGTTACTCTCAAGATAAAGCAGCAGAACTAATGGCTGATTTTAAGGCCGGAGCTGACGCTGTACTC
ACAAAGGTATCAGGCAGCCTTCCTGCAAATTTCCCAGCCCATATCAGCGATCCTATTTTTGATGGTATTCGCCGCCAGAC
AAATAAATTGCCGGGGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9537.77 Da
• Isoelectric Point: 8.8285

>AT23811 WP_015880151.1 NC_012691:3413318-3413569 [Tolumonas auensis DSM 9187]
MRITSPQMLSQALRDARIQRKLSQQATARQTGMKQTTVSAFENHPDGTRIETLFKLLSALDLELHLAKRDSEPDKSQGWD
QEW

Nucleotide

• Length: 252 bp

>AT23811 NC_012691:3413318-3413569 [Tolumonas auensis DSM 9187]
ATGCGGATTACCTCACCTCAAATGCTTTCTCAAGCTCTACGCGATGCCCGTATACAACGAAAACTGAGTCAACAGGCAAC
GGCCAGACAGACAGGAATGAAGCAAACGACTGTTTCTGCATTTGAAAACCACCCTGATGGAACCAGAATAGAAACATTGT
TCAAACTACTTTCGGCATTGGATCTGGAACTGCATCTGGCTAAACGCGACAGCGAACCGGATAAGTCTCAGGGGTGGGAT
CAGGAATGGTAG

Structures

Toxin

• AlphaFold DB: C4LDU2

Antitoxin

• AlphaFold DB: C4LDU1