Detailed information of TA system TA023810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 3128333..3128861
• Replicon: chromosome
• Accession: NC_012691
• Organism: Tolumonas auensis DSM 9187

Toxin (Protein)

• Gene name: relE
• Uniprot ID: C4LCD9
• Locus tag: TOLA_RS14610
• Protein ID: WP_015879892.1
• Coordinates: 3128571..3128861 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C4LCD8
• Locus tag: TOLA_RS14605
• Protein ID: WP_015879891.1
• Coordinates: 3128333..3128581 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOLA_RS14565	3123617..3124438	-	822	WP_015879883.1	hypothetical protein	-
TOLA_RS14570	3124573..3125739	-	1167	WP_015879884.1	nucleotide sugar dehydrogenase	-
TOLA_RS14575	3125994..3126257	-	264	WP_015879885.1	Txe/YoeB family addiction module toxin	-
TOLA_RS14580	3126254..3126496	-	243	WP_015879886.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
TOLA_RS14585	3126599..3126901	-	303	WP_015879887.1	type II toxin-antitoxin system RelE/ParE family toxin	-
TOLA_RS14590	3126889..3127146	-	258	WP_015879888.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
TOLA_RS14595	3127282..3127815	-	534	WP_015879889.1	GNAT family N-acetyltransferase	-
TOLA_RS14600	3127812..3128081	-	270	WP_015879890.1	DUF1778 domain-containing protein	-
TOLA_RS14605	3128333..3128581	+	249	WP_015879891.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
TOLA_RS14610	3128571..3128861	+	291	WP_015879892.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TOLA_RS16720	3129086..3129304	-	219	WP_015879893.1	hypothetical protein	-
TOLA_RS14615	3129451..3129915	-	465	WP_015879894.1	hypothetical protein	-
TOLA_RS14620	3129919..3130323	-	405	WP_015879895.1	helix-turn-helix transcriptional regulator	-
TOLA_RS16725	3130326..3130715	-	390	WP_148210457.1	hypothetical protein	-
TOLA_RS14630	3131020..3132357	-	1338	WP_015879896.1	phosphoglucosamine mutase	-
TOLA_RS14635	3132441..3133445	-	1005	WP_015879897.1	NAD-dependent epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3126254..3142214	15960

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11129.01 Da
• Isoelectric Point: 10.5016

>T23810 WP_015879892.1 NC_012691:3128571-3128861 [Tolumonas auensis DSM 9187]
MTYKLEFKKSAQKEWSKLGSTVREQLKKKLLERLENPHVPAAKLSGAENLYKIKLRQSGYRLVYQVEDGIITVIVLSVGK
RERSEVYKAAIHRIDQ

Nucleotide

• Length: 291 bp

>T23810 NC_012691:3128571-3128861 [Tolumonas auensis DSM 9187]
ATGACCTATAAATTAGAATTTAAAAAATCAGCCCAAAAAGAGTGGAGCAAGCTAGGTTCAACTGTTCGGGAGCAGTTGAA
AAAGAAACTGCTTGAGCGGTTGGAAAATCCGCATGTACCAGCAGCTAAGCTATCAGGAGCTGAAAATCTCTATAAAATCA
AATTGCGCCAGTCAGGTTATCGGTTGGTGTATCAAGTCGAAGACGGCATTATTACAGTTATTGTGCTATCGGTCGGAAAA
CGCGAAAGAAGCGAAGTTTATAAAGCCGCGATACACCGAATTGACCAATAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8990.29 Da
• Isoelectric Point: 3.9951

>AT23810 WP_015879891.1 NC_012691:3128333-3128581 [Tolumonas auensis DSM 9187]
MTARILADVAASITELKANPMKVVGSGYGEPVAVLNRNEPAFYCVPAEAYELLMDRLEDLELLAIARERQDEPSIAVDIN
DL

Nucleotide

• Length: 249 bp

>AT23810 NC_012691:3128333-3128581 [Tolumonas auensis DSM 9187]
ATGACAGCCAGAATTCTTGCTGATGTTGCAGCTAGCATAACCGAACTCAAGGCAAATCCAATGAAAGTGGTTGGTAGTGG
TTATGGCGAACCCGTTGCCGTATTAAATAGAAACGAACCCGCATTCTACTGTGTACCCGCTGAAGCTTATGAACTCTTGA
TGGATCGTCTTGAAGATCTGGAGTTGTTGGCAATAGCCAGAGAGCGTCAGGATGAGCCGAGTATCGCGGTAGACATCAAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: C4LCD9

Antitoxin

• AlphaFold DB: C4LCD8