Detailed information of TA system TA238099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5694356..5694875
• Replicon: chromosome
• Accession: NZ_CP093321
• Organism: Pseudomonas syringae CC440

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: N023_RS24670
• Protein ID: WP_024664379.1
• Coordinates: 5694356..5694646 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S6UWW5
• Locus tag: N023_RS24675
• Protein ID: WP_002551444.1
• Coordinates: 5694636..5694875 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N023_RS24630 (N023_24630)	5689465..5690157	+	693	WP_024664386.1	DUF4225 domain-containing protein	-
N023_RS24635 (N023_24635)	5690815..5691138	+	324	WP_024664384.1	type II toxin-antitoxin system RelE/ParE family toxin	-
N023_RS24640 (N023_24640)	5691114..5691479	+	366	WP_024664383.1	putative addiction module antidote protein	-
N023_RS24645 (N023_24645)	5691571..5692215	-	645	Protein_4851	histidine phosphatase family protein	-
N023_RS24650 (N023_24650)	5692315..5692656	-	342	WP_024664382.1	DUF5713 family protein	-
N023_RS24655 (N023_24655)	5692794..5693075	-	282	WP_024664381.1	hypothetical protein	-
N023_RS24660 (N023_24660)	5693389..5694126	+	738	WP_024664380.1	hypothetical protein	-
N023_RS24665 (N023_24665)	5694137..5694268	-	132	Protein_4855	GNAT family N-acetyltransferase	-
N023_RS24670 (N023_24670)	5694356..5694646	-	291	WP_024664379.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
N023_RS24675 (N023_24675)	5694636..5694875	-	240	WP_002551444.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
N023_RS24680 (N023_24680)	5695037..5695510	-	474	WP_110783500.1	GNAT family N-acetyltransferase	-
N023_RS24685 (N023_24685)	5695609..5696193	-	585	WP_024664284.1	ATP-binding protein	-
N023_RS24690 (N023_24690)	5696237..5696806	-	570	WP_024664283.1	GNAT family N-acetyltransferase	-
N023_RS24695 (N023_24695)	5696931..5697461	+	531	WP_024664282.1	hypothetical protein	-
N023_RS24700 (N023_24700)	5697499..5698773	-	1275	WP_024664281.1	OprD family porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11334.21 Da
• Isoelectric Point: 10.2579

>T238099 WP_024664379.1 NZ_CP093321:c5694646-5694356 [Pseudomonas syringae CC440]
MTYNLEFDARALKEWHKLGDTVRQQLKKKLATILVAPRVEANRLHALPDCYKIKLRSSGYRLFYQVIDQEVVVFVVAVDK
REREEVYRKAADRLGR

Nucleotide

• Length: 291 bp

>T238099 NZ_LR890206:c63778-63398 [Klebsiella pneumoniae]
ATGAGACATATATCACCGGAAGAACTTGTTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8955.34 Da
• Isoelectric Point: 4.6017

>AT238099 WP_002551444.1 NZ_CP093321:c5694875-5694636 [Pseudomonas syringae CC440]
MASINVRIDDDLKARAYLELEKLGVTPSELLRQTLQYVAERGQLPFKTVLMTEEDEALLATVRERLAAPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT238099 NZ_LR890206:c63999-63778 [Klebsiella pneumoniae]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A0K8M1G9