Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1005798..1006432 Replicon chromosome
Accession NZ_CP093321
Organism Pseudomonas syringae CC440

Toxin (Protein)


Gene name vapC Uniprot ID A0A193SM35
Locus tag N023_RS04465 Protein ID WP_003345997.1
Coordinates 1005798..1006202 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0P9J133
Locus tag N023_RS04470 Protein ID WP_003421652.1
Coordinates 1006202..1006432 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N023_RS04435 (N023_04435) 1001581..1002063 + 483 WP_003345989.1 PAS domain-containing protein -
N023_RS04440 (N023_04440) 1002160..1003416 + 1257 WP_024663315.1 3-phosphoshikimate 1-carboxyvinyltransferase -
N023_RS04445 (N023_04445) 1003439..1004101 - 663 WP_003421647.1 ChrR family anti-sigma-E factor -
N023_RS04450 (N023_04450) 1004101..1004613 - 513 WP_024665472.1 sigma-70 family RNA polymerase sigma factor -
N023_RS04455 (N023_04455) 1004849..1005037 - 189 WP_024665471.1 hypothetical protein -
N023_RS04460 (N023_04460) 1005036..1005611 + 576 WP_024665470.1 hypothetical protein -
N023_RS04465 (N023_04465) 1005798..1006202 - 405 WP_003345997.1 tRNA(fMet)-specific endonuclease VapC Toxin
N023_RS04470 (N023_04470) 1006202..1006432 - 231 WP_003421652.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
N023_RS04475 (N023_04475) 1006549..1007430 - 882 WP_024665469.1 aldose 1-epimerase -
N023_RS04480 (N023_04480) 1007405..1008376 - 972 WP_024665468.1 DMT family transporter -
N023_RS04485 (N023_04485) 1008461..1009741 - 1281 WP_003421655.1 TRAP transporter large permease -
N023_RS04490 (N023_04490) 1009742..1010269 - 528 WP_003408544.1 TRAP transporter small permease -
N023_RS04495 (N023_04495) 1010333..1011358 - 1026 WP_024665467.1 TRAP transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-125)

Antitoxin

SpoVT-AbrB domain

(7-34)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14946.21 Da        Isoelectric Point: 6.8620

>T238096 WP_003345997.1 NZ_CP093321:c1006202-1005798 [Pseudomonas syringae CC440]
MLKYMLDTNICIFTIKNKPTSVREAFNLHHGQLCISAITLMELVYGAEKSLSPERNLAVVEGFTARLEVLPYDSDAAAHT
GMIRAELARSGTPIGPYDQMIAGHARSLGLVVITNNQREFRRVEGLRVEDWVSQ

Download         Length: 405 bp

>T238096 NZ_LR890204:c4834923-4834006 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGTTGAAGTGCTGAGTCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8508.52 Da        Isoelectric Point: 4.7247

>AT238096 WP_003421652.1 NZ_CP093321:c1006432-1006202 [Pseudomonas syringae CC440]
MEQSTVFKSNRSQAVRLPKAVALPDDVKRVDVVAVGRTRIISPAGEMWNSWFDGESVSDDFMAERGQPAEQQRESL

Download         Length: 231 bp

>AT238096 NZ_LR890204:c4835687-4834929 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A193SM35

Antitoxin

Source ID Structure
AlphaFold DB A0A0P9J133

References