Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4245727..4246224 Replicon chromosome
Accession NZ_CP093317
Organism Escherichia coli strain HUM3355

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MLA23_RS20495 Protein ID WP_001173343.1
Coordinates 4245727..4245900 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MLA23_RS20500 Protein ID WP_000398619.1
Coordinates 4245928..4246224 (-) Length 99 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MLA23_RS20485 (4242392) 4242392..4243849 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
MLA23_RS20490 (4244086) 4244086..4245603 + 1518 WP_001295074.1 lysine--tRNA ligase -
MLA23_RS20495 (4245727) 4245727..4245900 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MLA23_RS20500 (4245928) 4245928..4246224 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MLA23_RS20505 (4246451) 4246451..4246723 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MLA23_RS20510 (4246735) 4246735..4246965 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
MLA23_RS20515 (4247146) 4247146..4248777 + 1632 WP_001216477.1 sensor histidine kinase -
MLA23_RS20520 (4248774) 4248774..4249493 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-57)

Antitoxin

(5-94)


Sequences


Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T238088 WP_001173343.1 NZ_CP093317:c4245900-4245727 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T238088 NZ_LR890204:349018-349365 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATTCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT238088 WP_000398619.1 NZ_CP093317:c4246224-4245928 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT238088 NZ_LR890204:349365-349664 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41


Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References