Detailed information of TA system TA238067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53667..54403
• Replicon: plasmid pHUM7199_A
• Accession: NZ_CP093315
• Organism: Klebsiella pneumoniae strain HUM7199

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: MLA24_RS25135
• Protein ID: WP_003026803.1
• Coordinates: 53921..54403 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: MLA24_RS25130
• Protein ID: WP_003026799.1
• Coordinates: 53667..53933 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLA24_RS25085 (MLA24_25055)	49729..50091	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
MLA24_RS25090 (MLA24_25060)	50141..50491	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
MLA24_RS25095 (MLA24_25065)	50849..51118	+	270	WP_004152102.1	hypothetical protein	-
MLA24_RS25100 (MLA24_25070)	51106..51681	+	576	WP_004152103.1	hypothetical protein	-
MLA24_RS25105 (MLA24_25075)	51712..52206	+	495	WP_004152104.1	DNA-binding protein	-
MLA24_RS25110 (MLA24_25080)	52250..52618	+	369	WP_004152105.1	hypothetical protein	-
MLA24_RS25115 (MLA24_25085)	52652..52855	+	204	WP_004152106.1	HHA domain-containing protein	-
MLA24_RS25120 (MLA24_25090)	52904..53161	+	258	WP_004152107.1	hypothetical protein	-
MLA24_RS25125 (MLA24_25095)	53237..53491	+	255	WP_004152108.1	hypothetical protein	-
MLA24_RS25130 (MLA24_25100)	53667..53933	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
MLA24_RS25135 (MLA24_25105)	53921..54403	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
MLA24_RS25140 (MLA24_25110)	54615..55961	+	1347	WP_020314316.1	ISNCY family transposase	-
MLA24_RS25145	56122..56253	+	132	WP_004218042.1	hypothetical protein	-
MLA24_RS25150 (MLA24_25115)	56462..57627	-	1166	Protein_59	IS3 family transposase	-
MLA24_RS25155 (MLA24_25120)	57804..58766	-	963	WP_004152113.1	zinc metalloprotease HtpX	-
MLA24_RS25160 (MLA24_25125)	58753..59241	-	489	WP_004152114.1	phosphate-starvation-inducible PsiE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSCO-1 / aac(3)-IIa / blaTEM-1B / blaCTX-M-15	-	1..231078	231078

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T238067 WP_003026803.1 NZ_CP093315:53921-54403 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T238067 NZ_LR890199:c73229-73071 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT238067 WP_003026799.1 NZ_CP093315:53667-53933 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT238067 NZ_LR890199:c73400-73273 [Escherichia coli]
CGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTAA
GTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66