Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5028026..5028651 Replicon chromosome
Accession NZ_CP093314
Organism Klebsiella pneumoniae strain HUM7199

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MLA24_RS24490 Protein ID WP_002882817.1
Coordinates 5028026..5028409 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MLA24_RS24495 Protein ID WP_004150355.1
Coordinates 5028409..5028651 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MLA24_RS24475 (5025392) 5025392..5026294 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MLA24_RS24480 (5026291) 5026291..5026926 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MLA24_RS24485 (5026923) 5026923..5027852 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MLA24_RS24490 (5028026) 5028026..5028409 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MLA24_RS24495 (5028409) 5028409..5028651 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MLA24_RS24500 (5028856) 5028856..5029773 + 918 WP_009484979.1 alpha/beta hydrolase -
MLA24_RS24505 (5029787) 5029787..5030729 - 943 Protein_4782 fatty acid biosynthesis protein FabY -
MLA24_RS24510 (5030774) 5030774..5031211 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MLA24_RS24515 (5031208) 5031208..5032068 - 861 WP_004146232.1 virulence factor BrkB family protein -
MLA24_RS24520 (5032062) 5032062..5032661 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T238066 WP_002882817.1 NZ_CP093314:c5028409-5028026 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T238066 NZ_LR890199:c48496-48362 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT238066 WP_004150355.1 NZ_CP093314:c5028651-5028409 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT238066 NZ_LR890199:48541-48601 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References