Detailed information of TA system TA238064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4543607..4544123
• Replicon: chromosome
• Accession: NZ_CP093314
• Organism: Klebsiella pneumoniae strain HUM7199

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R4YAY3
• Locus tag: MLA24_RS22180
• Protein ID: WP_004178374.1
• Coordinates: 4543607..4543891 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A919M0P8
• Locus tag: MLA24_RS22185
• Protein ID: WP_032434351.1
• Coordinates: 4543881..4544123 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLA24_RS22155 (4539090)	4539090..4539353	-	264	WP_014908078.1	PTS sugar transporter subunit IIB	-
MLA24_RS22160 (4539483)	4539483..4539656	+	174	WP_032414379.1	hypothetical protein	-
MLA24_RS22165 (4539659)	4539659..4540402	+	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
MLA24_RS22170 (4540759)	4540759..4542897	+	2139	WP_004186701.1	anaerobic ribonucleoside-triphosphate reductase	-
MLA24_RS22175 (4543139)	4543139..4543603	+	465	WP_004222151.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
MLA24_RS22180 (4543607)	4543607..4543891	-	285	WP_004178374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MLA24_RS22185 (4543881)	4543881..4544123	-	243	WP_032434351.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MLA24_RS22190 (4544201)	4544201..4546111	-	1911	WP_004152270.1	PRD domain-containing protein	-
MLA24_RS22195 (4546134)	4546134..4547288	-	1155	WP_002886900.1	lactonase family protein	-
MLA24_RS22200 (4547354)	4547354..4548094	-	741	WP_002886899.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-68)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11155.97 Da
• Isoelectric Point: 10.3787

>T238064 WP_004178374.1 NZ_CP093314:c4543891-4543607 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T238064 NZ_LR890198:c4878674-4878363 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9003.47 Da
• Isoelectric Point: 6.3340

>AT238064 WP_032434351.1 NZ_CP093314:c4544123-4543881 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGGKVSLDDL

Nucleotide

• Length: 243 bp

>AT238064 NZ_LR890198:c4878362-4878072 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A6THG1