Detailed information of TA system TA238038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 3100107..3100667
• Replicon: chromosome
• Accession: NZ_CP093302
• Organism: Alkalihalobacillus sp. LMS6

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: MM326_RS16890
• Protein ID: WP_099303522.1
• Coordinates: 3100107..3100457 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: MM326_RS16895
• Protein ID: WP_099303524.1
• Coordinates: 3100461..3100667 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MM326_RS16855 (MM326_16785)	3095694..3096479	-	786	WP_099303509.1	RNA polymerase sigma factor SigB	-
MM326_RS16860 (MM326_16790)	3096445..3096924	-	480	WP_099303511.1	anti-sigma B factor RsbW	-
MM326_RS16865 (MM326_16795)	3096921..3097247	-	327	WP_099303514.1	STAS domain-containing protein	-
MM326_RS16870 (MM326_16800)	3097314..3098330	-	1017	WP_099303516.1	PP2C family protein-serine/threonine phosphatase	-
MM326_RS16875 (MM326_16805)	3098343..3098759	-	417	WP_099303518.1	anti-sigma regulatory factor	-
MM326_RS16880 (MM326_16810)	3098762..3099118	-	357	WP_035398810.1	STAS domain-containing protein	-
MM326_RS16885 (MM326_16815)	3099138..3099956	-	819	WP_099303520.1	STAS domain-containing protein	-
MM326_RS16890 (MM326_16820)	3100107..3100457	-	351	WP_099303522.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MM326_RS16895 (MM326_16825)	3100461..3100667	-	207	WP_099303524.1	antitoxin	Antitoxin
MM326_RS16900 (MM326_16830)	3101014..3102036	-	1023	WP_099303526.1	outer membrane lipoprotein carrier protein LolA	-
MM326_RS16905 (MM326_16835)	3102152..3102514	-	363	WP_099303528.1	holo-ACP synthase	-
MM326_RS16910 (MM326_16840)	3102605..3103357	+	753	WP_255223853.1	rhomboid family intramembrane serine protease	-
MM326_RS16915 (MM326_16845)	3103464..3104063	+	600	WP_255223854.1	YitT family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12887.00 Da
• Isoelectric Point: 8.5683

>T238038 WP_099303522.1 NZ_CP093302:c3100457-3100107 [Alkalihalobacillus sp. LMS6]
MIVKRGDVYFADLSPVVGSEQGGVRPVLIIQNNIGNRFSPTVIVAAITAQIQKAKLPTHVEINASKYGFDRDSVILLEQL
RTIDKQRLTDKITHLDEKMMHSVDKALTISLGLIDF

Nucleotide

• Length: 351 bp

>T238038 NZ_LR890189:c5152463-5152080 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGCCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7822.83 Da
• Isoelectric Point: 4.3351

>AT238038 WP_099303524.1 NZ_CP093302:c3100667-3100461 [Alkalihalobacillus sp. LMS6]
MNEVDTVLEQEKWNDPRLKDRAELKAEIRESMQQGYMEMAKINLTIASEAFLAEEEANHTLTRLVSGV

Nucleotide

• Length: 207 bp

>AT238038 NZ_LR890189:c5152705-5152463 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA